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wiki:documentation:monika:automated_pumping_analysis_-_ideal_case [2020/02/18 07:23]
mscholz [After pharaglow tracking analysis]
wiki:documentation:monika:automated_pumping_analysis_-_ideal_case [2020/02/21 07:47] (current)
mscholz [After pharaglow tracking analysis]
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 {{ :wiki:documentation:monika:2xmagnification_from_bulb.png?400 |}} {{ :wiki:documentation:monika:2xmagnification_from_bulb.png?400 |}}
  
 +The automated analysis is dependent on peak detection. Therefore, if we increase the apparent peak height of the trace, we can get very similar results from the difference image, the anterior part of the straight image and the posterior part!
 +{{ :wiki:documentation:monika:2xmag_linescananalysis.png?400 |}}
 +
 +The left 3 panels show the (median(line) - mean(line) for the anterior and posterior part, the the mean difference of the image (subtracted the normalized velocity to correct drifts). All three when rescaled give very very similar pumping rates (ca. 4Hz, right upper panel.) The peak shape is also quite consistent for anterior and posterior.
 ===== Brightfield tracking ===== ===== Brightfield tracking =====
 For animals in the lawn, the bulb is clearly visible at 2x magnification and pumps can be counted. However, when they move outside and faster, we loose sight of the bulb. I do not believe this is as easy as the fluorescence assay, but we can keep it in mind for single-worm imaging with a tracking stage. For animals in the lawn, the bulb is clearly visible at 2x magnification and pumps can be counted. However, when they move outside and faster, we loose sight of the bulb. I do not believe this is as easy as the fluorescence assay, but we can keep it in mind for single-worm imaging with a tracking stage.
wiki/documentation/monika/automated_pumping_analysis_-_ideal_case.txt ยท Last modified: 2020/02/21 07:47 by mscholz

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