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 wiki:documentation:monika:manual_validation_of_1x_freely_moving_pumping_experiments [2020/03/11 00:40]mscholz created wiki:documentation:monika:manual_validation_of_1x_freely_moving_pumping_experiments [2020/03/11 00:45] (current)mscholz [Worm 4] 2020/03/11 00:45 mscholz [Worm 4] 2020/03/11 00:40 mscholz created 2020/03/11 00:45 mscholz [Worm 4] 2020/03/11 00:40 mscholz created Line 22: Line 22: (C) Correlation between automated and manual pumping rate. The black line shows unity, and the red and blue line show the expected error ranges for different frequencies based on a the manual annotator deviating its pumps by a single frame. This effect becomes pronounced at large pumping rates. e.g., if there are true pumps 5 frames apart, this would lead to a rate of 30fps/5frames = 6Hz. However, if the manual annotator deviated by 1 frame to result in the peaks being 6 frames apart, the frquency would be 30 fps/6 = 5Hz.\\ (C) Correlation between automated and manual pumping rate. The black line shows unity, and the red and blue line show the expected error ranges for different frequencies based on a the manual annotator deviating its pumps by a single frame. This effect becomes pronounced at large pumping rates. e.g., if there are true pumps 5 frames apart, this would lead to a rate of 30fps/5frames = 6Hz. However, if the manual annotator deviated by 1 frame to result in the peaks being 6 frames apart, the frquency would be 30 fps/6 = 5Hz.\\ (D) Histogram of pumping rates. \\ (D) Histogram of pumping rates. \\ - (E) velocity as the worm enters the lawn. The dashed line denotes lawn entry. + (E) velocity as the worm enters the lawn. The dashed line denotes lawn entry.\\ (F) Instantaneous deviations in pumps per 0.5s bin (black) and cumulative deviation of detected pumps from manual pumps. (F) Instantaneous deviations in pumps per 0.5s bin (black) and cumulative deviation of detected pumps from manual pumps.