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wiki:documentation:plasmids

Plasmids

What to do when you generate a new plasmid

1) Give it a unique plasmid name, such as pLJ# (p stands for plasmids, and LJ is the initial. # should be in numeric order), use your own initials for the plasmids you made.

2) Write details about how you create this plasmid

  • a. Include primers and template you used.
  • b. Write down the vector backbone.
  • c. Indicate whether it has been sequenced (which you always should) and a summary of sequencing result (such as whether it is 100% wt, or contains silent mutations). Indicate where the sequencing result is stored.

3) Enter into the database on wiki and indicate the antibiotic resistance.

4) Draw a plasmid map using SNAPgene software

Example: you generate a plasmid which is lite-1p::YFP
Example of details: Primer pair xxx and xxx are used to amplify lite-1 promoter from N2 genomic DNA (3kb). Primer pair xxx and xxx are used to amplify YFP from plasmid pMS1 (0.7kb). They are cloned into the vector xxx through Gibson Assembly. Primers xxx are used for sequencing. lite-1 promoter region is 100% correct, while there is a silent mutation at position xxx for YFP. Sequencing result can be found at xxx.

wiki/documentation/plasmids.txt · Last modified: 2019/11/15 17:12 by mscholz

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