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wiki:documentation:strains

Worm strains

Categorized Hermaphrodite sensory receptors

What to do when you generate a new strain

1) Allele names must be clearly indicated.
2) If you create a new transgene or knock-out or insertion, give it a new allele name
3) Assign a new strain name
4) Write details about how you create this strain

  • If it comes from a genetic cross, write the strain names of the “father” and “mother”, and how you genotype them for homozygosity. If the genotyping requires PCR (or sequencing), indicate what primers you use, expected band size, and where to find the gel picture (or sequencing result).
  • If it comes from a transgene, write the plasmid name, co-injection marker, concentration of the plasmids, and the strain that has been injected.

5) Enter into the database on wiki, give it a documentation #, which has to be in numeric order!!!
6) Write Jun an email about the update and he will proofread.

Example 1 (phenotyping based on phenotype): you created dpy-10(e128) II; wtfIs256 [myo-2p::mCherry]
Example details 1: AML283 males are crossed to CB128 dpy-10(e128). In the F2s, the plate (#2) that contains 100% pharyngeal mCherry and 100% dumpy are chosen. This new strain is named as INFxxx.

Example 2 (phenotyping based on PCR): you created eat-2(ad1113) II; zwEx104 [inx-4p::GFP + lin-15(+)]
Example details 2: HW2679 eat-2(ad1113) males are crossed to ZW284 GFP hermaphrodites. In the F2s, primer pair (xxx and xxx) are used to genotype for eat-2(ad1113). Expected size is 0.5kb. Primer xxx is used for sequencing. Plate #3 contains the zwEx104 arrays and is homozygous for eat-2(ad1113). Sequencing results can be seen at xxx. This new strain is named as INFxxx.

Backup database for transgenic worms

Documentation # Strain Name (geno) Type Description Location Source Notes Dewer Stock
1 MT17641 nIs264[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765) X I1 marker, backcrossed 3x. Jun's note: lin-15(n765) seems to be outcrossed already. Box 1, Row 1 Horvitz (MIT) I1, AWC, AWB expression Basket 1, #1 28-11-19 9 tubes
2 MT17912 nIs282[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765) X on Chromosome I, backcrossed 3 times to N2 Box 1, Row 2 Horvitz (MIT) I1, AWC, AWB expression Basket 1, #1 28-11-19 9 tubes
3 MT21198 nEx1997[gpa-16 prom ::gfp; unc-54 prom ::rfp] (now gcy-10 is odr-1) ??? paper says gpa-16 promoter but datasheet says gcy-10 Box 1, Row 3 Horvitz (MIT) maintain by picking non-muv Basket 1, #1 25-11-19 3 tubes, 29-11-19 6 tubes Tested for 25-11-19 batch
4 MT21419 nIs560[gcy-10 prom ::enphr3::yfp; lin-15(+)]; lin-15(n765) YFP in AWC/I1 (faint) Box 1, Row 4 Horvitz (MIT) Halorhodopsin in I1 Basket 1, #1 25-11-19 3 tubes, 29-11-19 6 tubes Tested for 25-11-19 batch
5 MT23561 nIs552[gcy-10 prom ::chr2::yfp; lin-15(+)]; lite-1(ce314) gur-3(ok2245) lin-15(n765) ChR in I1, AWC, AWB Box 1, Row 5 Horvitz (MIT) used by Nikhil in paper Basket 1, #2 28-11-19 9 tubes
6 NL2334 pkIs1273[gpa-16 prom ::gfp] GFP in gpa-16 Box 1, Row 6 Horvitz (MIT) super faint GFP in RIP Basket 1, #2 25-11-19 3 tubes, 29-11-19 6 tubes Tested for 25-11-19 batch
7 ZW284 zwEx104 [inx-4p::GFP + lin-15(+)] GFP in ADA, ADE, AIN, AUA, Box 1, Row 7 CGC Basket 1, #2
AVJ, DVC, FLP, PHA, PHB, PVR, PVT, RIC, RIG, RIM, RIP Basket 1, #2
8 BC11358 pdfr-1::gfp (sIs10334 [rCesC13B9.4::GFP + pCeh361]) Box 1, Row 8 Horvitz (MIT) Basket 1, #3
9 AML281 unc-31 (DA509; AML32) Box 1, Row 9 unc on food, whole-brain strain Basket 1, #3 26-11-19 3 tubes, 28-11-19 6 tubes Tested for 25-11-19 batch
10 AML282 unc-31 (MT2940; AML32) Box 2, Row 1 unc on food, whole-brain strain Basket 1, #3
11 PS5647 unc-119(ed4) III; him-5(e1490) V; syIs202. Box 2, Row 2 syIs202 [vang-1::YFP + myo-2::DsRed + unc-119(+)]; outcrossing suggests array is integrated in LG V. Reference: Green JL, et al. Cell. 2008 Aug 22;134(4):646-56. Basket 1, #3 29-11-19 9 tubes
12 AML283 wtfIs256 [myo-2::mCherry] mCherry in Pharynx, outcrossed 6x Box 2, Row 3 Basket 1, #4 29-11-19 9 tubes
13 AML284 wtfIs257 [myo-2::mCherry] mCherry in Pharynx, outcrossed 6x Box 2, Row 4 Basket 1, #4 28-11-19 9 tubes
14 AML327 pha-1(e2123)III; wtfEx275 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX] ehs-1 is supposed to be pan-pharyngel neural expression Box 2, Row 5 expression only in larvae Basket 1, #4
15 AML328 pha-1(e2123)III; wtfEx276 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX] ehs-1 is supposed to be pan-pharyngel neural expression Box 2, Row 6 expression only in larvae Basket 1, #4
16 AML32 wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons Box 2, Row 7 CGC Basket 1, #4
17 AML18 wtfIs3 [rab-3p::NLS::GFP + rab-3p::NLS::tagRFP]. RFP and GFP expression in the nuclei of all neurons. AML18 acts as a control for the calcium imaging strain AML14 Box 2, Row 8 CGC Basket 1, #5
18 CZ23281 juEx7105 [mec-4p::PH::miniSOG(Q103L) + mec-4p::mCherry + ttx-3::RFP] Pick RFP+ to maintain Box 2, Row 9 CGC Basket 1, #5
19 OH15262 otIs669 V NeuroPal Box 3, Row 1 CGC Basket 1, #5
20 OH15265 otIs672 [rab-3::NLS::GCaMP6s + arrd-4:NLS:::GCaMP6s] Bright panneuronal nuclear GCaMP6s expression Box 3, Row 2 CGC dropped during transfering
21 OH15500 otIs669 V; otIs672 Box 3, Row 3 CGC Basket 1, #5
22 OH16230 otIs670 V; otIs672 Box 3, Row 4 CGC Basket 1, #6
23 OH16290 otIs670 V; wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP] Box 3, Row 5 CGC Basket 1, #6
24 SD1614 ccIs4251 I; stIs10447 ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10447 [ceh-34p::HIS-24::mCherry + unc-119(+)]. Box 3, Row 6 CGC Basket 1, #6
25 ST2 ncIs2 [pH20::GFP + pBlueScript] II Expresses GFP in nearly all neurons Box 3, Row 7 CGC Basket 1, #6
26 BR5602 tax-4(p678) III; byEx836 [odr-4p::tax-4::GFP + myo-2p::mCherry] Box 3, Row 8 CGC Basket 1, #6 08-01-2020 9 tubes
27 BW1946 ctIs43 unc-42(e270) V. Box 3, Row 9 CGC Basket 1, #7
28 GRU101 gnaIs1 [myo-2p::yfp] IV Expresses YFP in pharynx, very likely on LGIV Box 4, Row 1 CGC Basket 1, #7 2020-02-05 9 tubes
29 HA329 lin-15B&lin-15A(n765) X; rtEx234 [nlp-13p::GFP + lin-15(+)] LGIV Box 4, Row 2 CGC Raise at 20C or higher and pick non-Muv or GFP+ to maintain array. Basket 1, #7 07-01-2020 9 tubes
30 HA341 lin-15B&lin-15A(n765) X; rtEx235 [nlp-3p::GFP + lin-15(+)]. Box 4, Row 3 CGC Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain. Basket 1, #7 08-01-2020 9 tubes
31 JN1713 peIs1713 [sra-6p::mCasp-1 + unc-122p::mCherry] ASH neurons are eliminated. Abnormal odor chemotaxis Box 4, Row 4 CGC Basket 1, #7
32 JN1715 peIs1715 [str-1p::mCasp-1 + unc-122p::GFP]. AWB neurons are eliminated. Abnormal odor chemotaxis Box 4, Row 5 CGC Basket 1, #8
33 LP177 unc-119(ed3) III; che-12(cp26[GFP + LoxP + unc-119(+) +LoxP]) V. Entire che-12 coding sequence deleted and replaced with GFP. Box 4, Row 6 CGC Basket 1, #8 09-01-2020 9 tubes
34 LX929  vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. Box 4, Row 7 CGC Basket 1, #8 08-01-2020 9 tubes
35 MH2805  kuIs70 [alr-1p::GFP + rol-6(su1006)]. Rollers. alr-1p::GFP expression is visible in embryonic and adult tissues. Box 4, Row 8 CGC Basket 1, #8 08-01-2020 9 tubes
36 MT19454 nIs396 [sams-5 3'::4xNLS-GFP + lin-15(+)] V.  GFP expression in MI Box 4, Row 9 CGC Basket 1, #8 07-01-2020 9 tubes
37 NH2466  ayIs4 I; dpy-20(e1282) IV.  Box 5, Row 1 CGC Basket 1, #9 07-01-2020 9 tubes
38 NY2045 ynIs45 [flp-15p::GFP] I; him-5(e1490) V. Box 5, Row 2 CGC Basket 1, #9
39 NY2049 ynIs49 [flp-5p::GFP]. Box 5, Row 3 CGC Basket 1, #9 2020-02-05 9 tubes
40 NY2064  ynIs64 [flp-17p::GFP] I; him-5(e1490) V. Box 5, Row 4 CGC Basket 1, #9
41 OH14372 pha-1(e2123) III; him-5(e1490) V; otEx6713 [str-97p::GFP + pha-1(+)]. Box 5, Row 5 CGC Maintain at 25C to select for transgenic array.  Basket 1, #9 09-01-2020 9 tubes
42 PS3504  syIs54 [ceh-2::GFP + unc-119(+)]; unc-119(ed4) III. Box 5, Row 6 CGC Do not distribute this strain; other labs should request it from the CGC Basket 1, #10
43 PY2417 oyIs44 [odr-1::RFP + lin-15(+)]. Bright RFP in AWB and AWC. Box 5, Row 7 CGC Basket 1, #10 07-01-2020 9 tubes
44 VM123 lin-15B&lin-15A(n765) X; akEx29 [glr-8::GFP + lin-15(+)]. Box 5, Row 8 CGC Maintain by picking non-Muv. Worms with the array are non-Muv at 20C. Basket 1, #10 07-01-2020 9 tubes
45 VM149 lin-15B&lin-15A(n765) X; akEx38 [(pDM46) glr-7::GFP + (pJM23) lin-15(+)] Box 5, Row 9 CGC Worms with the array are non-Muv at 20C. Basket 1, #10 08-01-2020 9 tubes
46 TY4236; IC1204 mIs10; him-8 bright GFP in pharynx Box 6, Row 1 Ian Chin-Sang Basket 1, #10
47 IC722 mIs11; him-5 bright GFP in pharynx Box 6, Row 2 Ian Chin-Sang Basket 1, #10
48 IC314 zdIs5; him-5 GFP in 6 touch neurons Box 6, Row 3 Ian Chin-Sang Basket 1, #11
49 INF3 spp-12p::gfp; rol-6 as coinjection marker GFP in NSM and I6, roller worms. Box 6, Row 4 Matthias Leippe Basket 1, #11
50 ZX1917 zxEx942[pmyo2::QuasAr::mOrange; pmyo-3::CFP] Box 6, Row 5 Alexander Gottschalk Basket 1, #11
51 ZX1918 zxEx943[pmyo-2::MacQ::mCitrine; pmyo3::mCherry] Box 6, Row 6 Alexander Gottschalk Basket 1, #11
52 ZX1958 zxEx944[pmyo-2::ArchD95N::2xmyc-tag; pmyo3::CFP] Box 6, Row 7 Alexander Gottschalk Basket 1, #11
53 OK0765 cuEx613[dbl-1 M4 enhancerwild-type] Label M4 neuron, GFP Box 6, Row 8 Peter Okkema Pick rollers Basket 1, #12
54 LSC1428 trh-1(lst1118); lstEx776 [Pglr-8::trh-1::SL2::gfp, 50 ng/μL + pCIM02 (Punc-122::dsRED), 50 ng/μL]. Label M4 and M5 neurons Box 6, Row 9 Isabel Beets Pick GFP or RFP worms Basket 1, #12 10/23/2020
55 MT15670 nIs175 IV [ceh-28p::4xNLS::GFP + lin-15(+)] M4 marker Box 7, Row 1 Bob Horvitz Backcrossed 5 times Basket 1, #12
56 MT15672 nIs177 I [ceh-28p::4NLS::GFP + lin-15(+)]. M4 marker Box 7, Row 2 Bob Horvitz Backcrossed 5 times Basket 1, #12
57 MT17913 nIs283 X [gcy-10p::GFP + lin-15(+)] I1 marker Box 7, Row 3 Bob Horvitz Backcrossed 3 times Basket 1, #12
58 MT19378 nIs390 III [Pflp-15∷gfp, lin-15AB(+)] I2 marker Box 7, Row 4 Bob Horvitz Backcrossed 5 times Basket 1, #12
59 MT21910 lin-15AB(n765ts) X; nEx2065 [Pgur-3::gfp, lin-15(+)] Expressed in I2, I4, AVD and PVC Box 7, Row 5 Bob Horvitz lost
60 INF100 tax-2(p671); gnaIs1 [myo-2p::yfp] Box 7, Row 6 Nicolina Basket 2, #13
61 IC765 quEx180 [rde-1p::rfp] pick RFP to maintain Box 7, Row 7 Ian Chin-Sang Basket 2, #13
62 IC2226 quIs29 integrated rde-1p::rfp with rgef-1p::ins-4 Box 7, Row 8 Ian Chin-Sang Basket 2, #13
63 INF101 tax-4(p678); gnaIs1 [myo-2p::yfp] YFP in pharynx, defective chemotaxis Box 7, Row 9 Nicolina Basket 2, #13
64 INF102 unc-9(e101)X; gnaIsl[myo2-p::yfp] YFP in pharynx, unc Box 8, Row 1 Nicolina Basket 2, #13
65 INF103 pkc-1(nj1)V; gnaIsl[myo2-p::yfp] YFP in pharynx, defective chemotaxis to AWA- and AWC- odorants Box 8, Row 2 Nicolina Basket 2, #14
66 INF104 pels1715[str-1p::mCasp-1 + unc-122p::GFP]; gnasIsl[myo2-p::yfp] YFP in pharynx, GFP spots on underside, AWB eliminated Box 8, Row 3 Nicolina Nicolina did not manage to make this strain
67 SWF62 flvEx30[Ptph-1::GCaMP5a, Ptph-1::mCherry, Psrg-47:: GCaMP5a, Psrg-47::mCherry] Box 8, Row 4 Steven Flavell Basket 2, #14 Frozen, but heavily contaminated
68 CX15388 kyEx5178[tph-1(short)::HisCl1-sl2-mCherry] Box 8, Row 5 Steven Flavell Basket 2, #14
69 CX15485 unc-13(s69); lite-1(ce314); kyEx4656 [tph-1-NSMfragment::GCaMP5A; myo-3::mCherry] Box 8, Row 6 Steven Flavell Basket 2, #14
70 CX16651 kyIs694 [tph-1-NSM-fragment::rpl-22-3xHA; tph-1NSM-fragment::GFP] Box 8, Row 7 Steven Flavell Basket 2, #14
71 CZ20310 juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] unc-119(ed3) III. optogenetic strain for integration and mutagenesis, with miniSOG Box 8, Row 8 CGC Basket 2, #15
72 OH15363 otIs669 him-5(e1490) V NeuroPal with him-5 Box 8, Row 9 CGC Basket 2, #15
73 OH4141 zdIs21 IV; wrk-1(ok695) X Box 9, Row 1 CGC Basket 2, #15
74 PS6843 syIs300 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript].  V GFP cGAL effector.  Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with transgenetic marker in next generation.  Box 9, Row 2 CGC Backcrossed 7 times Basket 2, #15 10/23/2020
75 PS6844 syIs301 [myo-2p:NLS::GAL4SC::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] V. myo-2 cGAL driver for pharyngeal muscle. Note this is sc version. Should use sk version. Box 9, Row 3 CGC Backcrossed 5 times Basket 2, #15
76 PS6872 syIs302 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. III. GFP cGAL effector.  Box 9, Row 4 CGC Backcrossed 6 times Basket 2, #16
77 PS6963 syIs336 [rab-3p::NLS::GAL4SK::VP64::let-858 3'UTR + unc-122p::RFP +  pBlueScript]. X. rab-3 cGAL driver for the whole nervous system.  Box 9, Row 5 CGC Backcrossed 4 times Basket 2, #16
78 PS7185 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. IV. GFP::H2B effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.  Box 9, Row 6 CGC Backcrossed 3 times Basket 2, #16
79 PS7190 syIs409 [15xUAS::?pes-10::mCherry::H2B::let-858 3'UTR + unc-122p::GFP + pBlueScript] X. mCherry::H2B cGAL effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. Box 9, Row 7 CGC Backcrossed 2 times Basket 2, #16
80 PS7199 syIs371 [15xUAS::?pes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)] III. Histamine chloride channel cGAL effector. Box 9, Row 8 CGC Backcrossed 3 times Basket 2, #16
81 PS7200 syIs420 [15xUAS::?pes-10::tetx::let-858 3'UTR + myo-2p::NLS::GFP + pBlueScript] IV. Tetanus toxin cGAL effector. Box 9, Row 9 CGC Backcrossed 3 times Basket 2, #16
82 PS7203 syIs423 [15xUAS::?pes-10::GCaMP6s::SL2::mKate2::let-858 3'UTR + myo-2p::NLS::mCherry + 1kb DNA ladder(NEB)] V. GCaMP6s cGAL effector. Box 20, Row 1 CGC Backcrossed 3 times Basket 2, #17
83 INF1 ynIs45 [flp-15p::GFP] I; otIs669 V Box 20, Row 2 Jun OH15262 crossed to NY2045 Basket 2, #17
84 INF2 syIs54 [ceh-2::GFP + unc-119(+)] II; otIs669 V Box 20, Row 3 Jun OH15262 crossed to PS3504 Basket 2, #17
85 INF4 odr-4 (n2144) III; gnaIs1 IV Box 20, Row 4 Jun MT5300 cross to GRU101 Basket 2, #17
86 INF5 unc-31(e928) gnaIs1 IV Box 20, Row 5 Jun DA509 cross to GRU101 Basket 2, #17
87 JN3038 qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100 [H20p::nls4::mCherry]; qjIs14[H20p::nls::YC2.60 express fluorescent calcium indicator Yellow-Cameleon 2.60 with the pan-neuronal promoter H20p in the background of JN3039. Jun's note: seems to be sicker than JN3039. Box 20, Row 6 Gift from Yuichi Iino Basket 2, #18
88 JN3039 qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100 [H20p::nls4::mCherry] roiedit3D Neuron ID strain Box 20, Row 7 Gift from Yuichi Iino Basket 2, #18 21-07-20, 9 tubes
89 INF6 nonEx1[rab-3p::his-24::tagBFP; myo-2p::mCherry::unc-54 3'utr] To see the expression strength of rab-3p::his-24::tagBFP Box 20, Row 8 Jun iLJ3 line 1 Basket 2, #18 21-07-20, 9 tubes
90 INF7 nonEx2[rab-3p::his-24::tagBFP; myo-2p::mCherry::unc-54 3'utr] To see the expression strength of rab-3p::his-24::tagBFP Box 20, Row 9 Jun iLJ3 line 2 Basket 2, #18 21-07-20, 9 tubes
91 INF8 nonEx3[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 1 Box 21, Row 1 Jun iLJ4 line 1 Basket 2, #18
92 INF9 nonEx4[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 2 Box 21, Row 2 Jun iLJ4 line 2 Basket 2, #19
93 INF10 nonEx5[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 3 Box 21, Row 3 Jun iLJ4 line 3 Basket 2, #19
94 NY2030 ynIs30 [flp-4p::GFP] I Array seems prone to silencing. Check for GFP and pick GFP+ to maintain. Box 21, Row 4 CGC Basket 2, #19 26-08-20 9 tubes
95 NY2037 ynIs37 [flp-13p::GFP] III Box 21, Row 5 CGC Basket 2, #19 26-08-20 9 tubes; 01-09-20 refreeze
96 NY2080 ynIs80 [flp-21p::GFP]. Box 21, Row 6 CGC Basket 2, #19 26-08-20 9 tubes
97 PS7401 syIs433 IV; syIs300 V Pmyo-2 cGAL-C driver(syIs433). Co-injection marker for syIs433 is coel::RFP Box 21, Row 7 Gift from Paul Sternberg outcrossed x3 Basket 2, #20 2020-09-09 9 tubes
98 PS7402 syIs431 III; syIs300 V Pmyo-2::cGAL-N (syIs431). Co-injection marker for syIs431 is pmyo-2::NLS::mCherry Box 21, Row 8 Gift from Paul Sternberg outcrossed x3 Basket 2, #20 2020-09-09 9 tubes
99 PS6843 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL] GFP effector Box 21, Row 9 Gift from Paul Sternberg outcrossed x7 Basket 2, #20
100 PS7521 syIs483 X [Punc-17::cGAL-N, Pceh19b:: cGAL-C] Split cGAL drivers for MC neurons. Co-injection marker is coel::RFP Box 22, Row 1 Gift from Paul Sternberg outcrossed x3 Basket 2, #20 2020-09-09 9 tubes
101 PS7524 syIs371 III; syIs483 X Split MC driver (syIs483) > HisCl1 effector (syIs371, 15xUAS::HisCl1::SL2::gfp::let-858 3’UTR). Co-injection marker for syIs371 is coel::RFP Box 22, Row 2 Gift from Paul Sternberg Basket 2, #20 2020-09-09 9 tubes
102 PS7154 syIs391 IV [myo-2p::NLS::GAL4SK::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] myo-2 cGAL driver for pharyngeal muscle. Box 22, Row 3 Gift from Paul Sternberg Basket 2, #20 2020-08-31 9 tubes
103 INF12 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx4[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] pick pan-neuronal BFP to maintain Box 22, Row 4 Jun iLJ4 #2 in PS7185 background Basket 2, #21
104 INF13 nonEx6[pPHA2∷GFP-F + Pmyo-2::mCherry::unc-54utr] pick mCherry pharynx to maintain Box 22, Row 5 Jun iLJ6 #1 Basket 2, #21 26-08-20 9 tubes. 2020-12-30 refreeze, 2021-02-04 refreeze again
105 INF16 gnaIs1 IV; odr-7(ky4) X Box 22, Row 6 Jun Basket 2, #21 15-09-2020 9 tubes
106 INF17 unc-31(n1304) gnaIs1 IV Box 22, Row 7 Jun Basket 2, #21 15-09-2020 9 tubes
107 INF18 gnaIs1 IV; unc-9(fc16) unc-7(e5) X. Box 22, Row 8 Jun Basket 2, #21 25-09-2020 9 tubes, 07-10-2020 9 tubes
108 INF21 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx7[odr-1p short::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] odr-1p short in GAL4 driver line, directly injected in GFP effector line (PS7185). Line 1 Box 22, Row 9 Jun iLJ5 #1 Basket 2, #21 10/23/2020. 2020-12-30 refreeze
109 INF22 gnaIs1 IV; mec-4(u253) X note: this mec-4(u253) is from Martin Chalfie's lab Box 23, Row 1 Jun Basket 2, #22 2020-11-11 9 tubes
110 INF23 gnaIs1 IV; odr-1(n1936) X note: it seems hard to make the cross, perhaps synthetic unhealthy Box 23, Row 2 Jun Basket 2, #22 2020-11-11 9 tubes, 2020-12-03 refreeze
111 INF24 gnaIs1 IV; unc-9(fc16) X Box 23, Row 3 Jun Basket 2, #22 12/3/2020
112 INF25 gnaIs1 IV; mec-10(u332) X Box 23, Row 4 Jun Basket 2, #22 12/3/2020
113 INF26 gnaIs1 IV; unc-7(e5) X Box 23, Row 5 Jun empty 12/3/2020
114 INF29 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx8[odr-1p short::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] odr-1p short in GAL4 driver line, directly injected in GFP effector line (PS7185). Line 4 Box 23, Row 6 Jun iLJ5 #4 Basket 2, #23 12/30/2020
115 INF30 nonEx9[pPHA2∷GFP-F + Pmyo-2::mCherry::unc-54utr] pick mCherry pharynx to maintain Box 23, Row 7 Jun iLJ9 #1 Basket 2, #23 12/30/2020
116 INF31 nonEx10[Plin-44::NLS-tagBFP::let-858 3'UTR + Pmyo-2::mCherry::unc-54utr] pick mCherry pharynx to maintain Box 23, Row 8 Jun iLJ10 #2 Basket 2, #23 12/30/2020
117 INF32 nonEx11[Plin-44::NLS-tagBFP::let-858 3'UTR + Pmyo-2::mCherry::unc-54utr] pick mCherry pharynx to maintain Box 23, Row 9 Jun iLJ10 #3 Basket 2, #23 12/30/2020
118 INF33 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx12[Ptph-1::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] tph-1 in GAL4 driver line, directly injected in GFP effector line (PS7185). Line 1. pick rab-3p::bfp to maintain Box24, Row 1 Jun iLJ13 #1 Basket 2, #23 12/30/2020
119 INF34 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx13[Pceh-34::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::mScarlet] ceh-34 in GAL4 driver line, directly injected in GFP effector line (PS7185). Line 1. pick rab-3p::scarlet to maintain Box24, Row 2 Jun iLJ14 #1 Basket 2, #24 2/4/2021
120 INF35 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx14[Pceh-34::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::mScarlet] ceh-34 in GAL4 driver line, directly injected in GFP effector line (PS7185). Line 2. pick rab-3p::scarlet to maintain Box24, Row 3 Jun iLJ14 #2 Basket 2, #24 12/30/2020
121 AML1 zfIs18 [mec-4p::ChR2::YFP]; zfIs42 [rig-3p::GCaMP3::SL2::mCherry]. Box24, Row 4 CGC Basket 2, #24 2/4/2021
122 AQ3236 ljSi2 [mec-7::GCaMP6m::SL2::TagRFP + unc-119(+)] II; unc-119(ed3) III. Box24, Row 5 CGC Basket 2, #24 2/4/2021
123 CX17256 kyIs722 [str-2p::GCaMP5(D380Y) + elt-2::mCherry] Box24, Row 6 CGC Basket 2, #24 2/4/2021
124 DA1269 lin-15B&lin-15A(n765) X; adEx1269 [odr-1::GFP + lin-15(+)]. maintain by picking non-Muv Box24, Row 7 CGC Basket 3, #25 2/4/2021
125 EG8078 oxTi185 [ttTi5605 + NeoR(+) + unc-18(+)] I; unc-119(ed3) III. MosSCI line for Chromosome I Box24, Row 8 CGC Basket 3, #25 9/4/2021
126 EG8079 oxTi179 [ttTi5605 + NeoR(+) + unc-18(+)]; unc-119(ed3) III. MosSCI line for Chromosome II Box24, Row 9 CGC Basket 3, #25
127 QW625 zfIs42 [rig-3p::GCaMP3::SL2::mCherry + lin-15(+)] Box25, Row 1 CGC Basket 3, #25 2/4/2021
128 SRS86 sraIs49 [nmr-1p::G-CaMP + unc-119(+)] V; lite-1(ce314) X; sraEx83 [tdc-1p::chop-2(H134R)::mCherry + F55B11.3p::mCherry] Maintain by picking red fluorescent worms. Box25, Row 2 CGC Basket 3, #25
129 INF36 nIs264[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP] Box25, Row 3 Jun Cross MT17641 to AML32 Basket 3, #26 30-03-2021
130 INF37 gnaIs1; odr-10(ky32) X Box25, Row 4 Jun Basket 3, #26 30-03-2021
131 INF38 gnaIs1; odr-10(225) X Box25, Row 5 Jun Basket 3, #26 30-03-2021
132 PS6916 cGAL driver, nlp-40p cGAL driver: intestine Box25, Row 6 Gift from Paul Sternberg Basket 3, #26 5/6/2021 successful
133 PS7044 UAS::hChR2(H134R)::YFP IV cGAL effector Box25, Row 7 Gift from Paul Sternberg Basket 3, #26 5/6/2021 successful
134 PS8285 UAS::-GtACR2-SL2-GFP cGAL effector Box25, Row 8 Gift from Paul Sternberg Basket 3, #27 5/21/2021
135 PS8373 cGAL driver, mec-17p cGAL driver: ALM, PLM, AVM, PVM Box25, Row 9 Gift from Paul Sternberg Basket 3, #27 5/6/2021 successful
136 PS8412 UAS::-TeTX-SL2-mKate cGAL effector Tetanus toxin, acts by inhibiting neurotransmitter release. Probably AIY rfp as marker Box26, Row 1 Gift from Paul Sternberg Basket 3, #27 5/6/2021 successful
137 PS8423 UAS::-GCaMP7f-SL2-mKate cGAL effector: Latest GCaMP, improved SNR, fast kinetics Box26, Row 2 Gift from Paul Sternberg Basket 3, #27 5/21/2021
138 PS8459 UAS::-wrmScarlet cGAL effector: C. elegans optimized version of mScarlet Box26, Row 3 Gift from Paul Sternberg Basket 3, #27 5/6/2021 successful
139 PS8473 UAS::-GCaMP7b-SL2-mKate cGAL effector: Latest GCaMP, improved SNR, higher baseline fluorescence Box26, Row 4 Gift from Paul Sternberg Basket 3, #27 6/17/2021
140 PS8499 UAS::-ChR2(C128S)-EYFP cGAL effector: Stable step function ChR2 variant. Activated by blue, terminated by green light. Box26, Row 5 Gift from Paul Sternberg Basket 3, #28 5/6/2021 successful
141 PS8505 UAS::-miniSOG-130L-VAMP2-mCherry cGAL effector: Inhibits synaptic release by disrupting the function of synaptic vesicles Box26, Row 6 Gift from Paul Sternberg Basket 3, #28 5/6/2021 successful
142 PS8507 UAS::-hChR2 (C128S, D156A)-EYFP cGAL effector: Stabilized step function Opsins ChR2 variant Box26, Row 7 Gift from Paul Sternberg Basket 3, #28 5/6/2021 successful
143 PS8508 UAS::-SwiCHR-TS-EYFP cGAL effector: Step-Waveform Inhibitory Channelrhodopsin Box26, Row 8 Gift from Paul Sternberg Basket 3, #28 5/21/2021
144 PS8660 cGAL driver, tph-1p, pdf-1p cGAL driver: NSM Box26, Row 9 Gift from Paul Sternberg Basket 3, #28 5/21/2021
145 PS9036 cGAL driver, gcy-8p cGAL driver: AFD; probably coel::RFP as marker Box27, Row 1 Gift from Paul Sternberg PS8566 x N2 (x3) Basket 3, #29 5/6/2021 successful
146 PS9088 UAS::15xUAS::jGCaMP7s::SL2::mKate2 cGAL effector: Latest GCaMP, improved SNR, slow kinetics. Marker: probably RFP in head and tail Box27, Row 2 Gift from Paul Sternberg PS8419 x N2 (x3) Basket 3, #29 5/6/2021 successful
147 INF39 eat-2(ad1113) II; gnaIs1 IV Box27, Row 3 Jun cross GRU101 to HW2679 Basket 3, #29 6/17/2021
148 INF40 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]; nonEx15[Pceh-2::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] Box27, Row 4 Jun iLJ17, line 4 Basket 3, #29
149 INF41 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]; nonEx16[Pceh-2::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] Box27, Row 5 Jun iLJ17, line 6 Basket 3, #29
150 INF42 gnaIs1; nonEx17[Prab-3::unc-1(n494) cDNA + Prab-3::his-24::linker::wrmScarlet] To express unc-1 dominant negative pan-neuronally Box27, Row 6 Jun iLJ22, line 2 empty
151 INF43 gnaIs1; nonEx18[Prab-3::unc-1(n494) cDNA + Prab-3::his-24::linker::wrmScarlet] To express unc-1 dominant negative pan-neuronally Box27, Row 7 Jun iLJ22, line 8 Basket 3, #30
152 AML67 wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54]. Transgenic animals express light-gated ion channel Chrimson and a fluorescent protein mCherry in mechanosensory neurons. Reference: Liu M, et al. eLife 2018;7:e36419 DOI: 10.7554/eLife.36419. Box27, Row 8 CGC Basket 3, #30
153 OH15275 pha-1(e2123) III; otEx7106 [unc-7(fosmid WRM0627bH02)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array.  Box27, Row 9 CGC Basket 3, #30
154 OH15278 pha-1(e2123) III; otEx7109 [unc-9(fosmid WRM0611aH10)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array.  Box28, Row 1 CGC Basket 3, #30
155 OH15281 pha-1(e2123) III; otEx7112 [che-7(fosmid WRM0640dF02)::SL2::NLS::YFP::H2B + pha-1(+) + myo-2p::BFP]. Maintain at 25C or pick BFP+ to retain array.  Box28, Row 2 CGC Basket 3, #30
156 PS7192 syIs413 [15xUAS::?pes-10::ICE::let-858 3'UTR + unc-122p::GFP + pBlueScript] Human caspase ICE cGAL effector. Probably coel::GFP as marker Box28, Row 3 CGC Basket 3, #30
157 ZW127 zwIs106 [unc-9p::GFP + lin-15(+)] Box28, Row 4 CGC
158 INF44 eat-18(ad820) I; gnaIs1 IV Jun
159 INF45 eat-18(ad1110) I; gnaIs1 IV Jun
160 INF46 gnaIs1 IV; mec-10(tm1552) X Jun
161 INF47 Stephanie did not respond to give allele names Cross PS7154 to PS8423 Box28, Row 8 Monika
162 INF48 Stephanie did not respond to give allele names Cross PS9036 to PS7192 Box28, Row 9 Jun
163 INF49 Stephanie did not respond to give allele names Cross PS9036 to PS8412 Box29, Row 1 Jun
164 INF50 Stephanie did not respond to give allele names Cross PS9036 to PS9088 Box29, Row 2 Jun
165 INF51 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]; nonEx19[Pceh-34::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] Box29, Row 3 iLJ23, line 2
166 INF52 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]; nonEx20[Pceh-34::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] Box29, Row 4 iLJ23, line 3
167 INF53 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]; nonEx21[Pceh-34::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] Box29, Row 5 iLJ23, line 4
168 INF54 syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]; nonEx22[Pceh-34::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] Box29, Row 6 iLJ23, line 6

Backup mutant database (ie. non-transgenics)

Numbering # Strain Name (geno) Type Description Location Source Notes Dewer stock
NT1 CB4856 Hawaiian isolate of C. elegans Box 10, Row 1 Grosshans (FMI), HW2675 Basket 10, #101
NT2 N2 wt Box 10, Row 2 Grosshans (FMI) Basket 10, #101
NT3 HW2678 eat-2(ad1116) Box 10, Row 3 Grosshans (FMI) Basket 10, #101
NT4 HW2679 eat-2(ad1113) Box 10, Row 4 Grosshans (FMI) Basket 10, #101
NT5 CB101 unc-9(e101) X Box 10, Row 5 CGC
NT6 CB187 rol-6(e187) II Box 10, Row 6 CGC
NT7 CB1128 che-7(e1128) V Box 10, Row 7 CGC
NT8 DA509 unc-31(e928) IV Box 10, Row 8 CGC
NT9 MT2940 unc-31(n1304) IV Box 10, Row 9 CGC
NT10 MT6308 eat-4(ky5) III Box 11, Row 1 CGC
NT11 TQ1101 lite-1(xu7) X Box 11, Row 2 CGC
NT12 BR5514  tax-2(p671) I; tax-4(p678) III Box 11, Row 3 CGC 12/23/2019 08-01-2020 9 tubes
NT13 IK105 pkc-1(nj1) V. Box 11, Row 4 CGC 12/23/2019
NT14 MT5300 odr-4(n2144) III. Box 11, Row 5 CGC 12/23/2019
NT15 N2 C. elegans wild isolate. Box 11, Row 6 CGC 12/23/2019 08-01-2020 9 tubes
NT16 N2 (ancestral) C. elegans wild type (ancestral). Box 11, Row 7 CGC 12/23/2019
NT17 PR671  tax-2(p671) I. Box 11, Row 8 CGC 12/23/2019
NT18 PR678 tax-4(p678) III. Box 11, Row 9 CGC 12/23/2019 07-01-2020 9 tubes
NT19 PR691 tax-2(p691) I. Box 12, Row 1 CGC 12/23/2019 08-01-2020 9 tubes
NT20 VC3113 tax-4(ok3771) III. Box 12, Row 2 CGC 12/23/2019 08-01-2020 9 tubes
NT21 WM27 rde-1(ne219) V Box 12, Row 3 Ian Chin-Sang 3/5/2020 Basket 10, #101
NT22 CB5 unc-7(e5) X. Box 12, Row 4 CGC 2020-03-20 9 tubes
NT23 CX4 odr-7(ky4) X Box 12, Row 5 Busack, Inka 8/5/2020 Basket 10, #102
NT24 CX2065 odr-1(n1936) X. Box 12, Row 6 Busack, Inka 8/5/2020 Basket 10, #102
NT25 CB102 unc-10(e102) X. Box 12, Row 7 CGC 8/17/2020 26-08-20 9 tubes
NT26 CB120 unc-4(e120) II. Box 12, Row 8 CGC 8/17/2020 26-08-20 9 tubes
NT27 CW129 unc-9(fc16) X. Box 12, Row 9 CGC 8/17/2020
NT28 NM1657 unc-10(md1117) X Box 13, Row 1 CGC 8/17/2020 26-08-20 9 tubes
NT29 ZM3087 unc-9(fc16) unc-7(e5) X. Box 13, Row 2 CGC 8/17/2020 2020-09-09 9 tubes
NT30 INF11 unc-31(n1304) IV BC6 Backcrossed 6 times Box 13, Row 3 Jun 8/21/2020 9 tubes Basket 10, #102
NT31 INF14 odr-7(ky4) X BC6 Backcrossed 6 times Box 13, Row 4 Jun 8/31/2020 Basket 10, #102
NT32 INF15 odr-1(n1936) X. BC6 Backcrossed 6 times Box 13, Row 5 Jun 8/28/2020; 01-09-2020 refreeze Basket 10, #102
NT33 TU253 mec-4(u253) X Box 13, Row 6 CGC 9/22/2020
NT34 INF19 unc-10(md1117) X BC2 Box 13, Row 7 Jun Basket 10, #103
NT35 INF20 unc-10(e102) X BC1 Box 13, Row 8 Jun Basket 10, #103
NT36 TU253 (MC lab) mec-4(u253) X maybe backcrossed Box 13, Row 9 Martin Chalfie 2020-10 11/4/2020 Basket 10, #103
NT37 TU536 mec-10(u332) X maybe backcrossed Box 14, Row 1 Martin Chalfie 10/26/2020 11/4/2020 Basket 10, #103
NT38 INF27 unc-7(e5) X. backcrossed 5 times Box 14, Row 2 Jun 12/3/2020 Basket 10, #103
NT39 INF28 unc-9(fc16) X. backcrossed 5 times Box 14, Row 3 Jun 12/30/2020 Basket 10, #104
NT40 CX32 odr-10(ky32) X. Box 14, Row 4 CGC 2/4/2021
NT41 CX3410 odr-10(ky225) X. Box 14, Row 5 CGC 2/4/2021
NT42 DA1084 egl-30(ad806) egl, backup strain in case three alleles are required Box 14, Row 6 CGC 7/14/2021
NT43 DA823 egl-30(ad805) 100% egl Box 14, Row 7 CGC 7/14/2021
NT44 MT1434 egl-30(n686) reference allele Box 14, Row 8 CGC 7/14/2021
NT45 DG2913 egl-30(ad810)/hT2 balanced null (homo lethal) Box 14, Row 9 CGC 7/14/2021
NT46 CE1047 egl-30(ep271) gain of function, but cannot be used on human subjects Box 15, Row 1 CGC 7/14/2021
NT47 PS2627 dgk-1(sy428) previously called sag-1, suppressor of egl-30, but cannot be used on human subjects Box 15, Row 2 CGC
NT48 JT609 eat-16(sa609) eat-16(sy438) suppresses egl-30, but this allele is not available from CGC. Box 15, Row 3 CGC 7/14/2021
NT49 MT1088 unc-1(n494) dominant negative mutant of unc-1 Box 15, Row 4 CGC 7/14/2021
NT50 VC2633 F25D1.4(ok3307) harsh touch defective. PMID: 20512132 Box 15, Row 5 CGC 7/14/2021
NT51 TQ296 trp-4(sy695) posterior harsh touch. PMID: 21587232 Box 15, Row 6 CGC 7/14/2021
NT52 CB1515 mec-10(e1515) X wt, do not use Box 15, Row 7 Ithai Rabinowitch 7/26/2021
NT53 ZB2551 mec-10(tm1552) X Box 15, Row 8 Ithai Rabinowitch 7/26/2021
NT54 DA1110 eat-18(ad1110) I. Box 15, Row 9 CGC 7/20/2021
NT55 DA820 eat-18(ad820) I. Box 16, Row 1 CGC 7/20/2021
wiki/documentation/strains.txt · Last modified: 2021/10/28 01:50 by jliu

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