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Editing the wiki
About the lab
Protocols
Money
Lab Notebook
Writing
Documentation
1) Allele names must be clearly indicated.
2) If you create a new transgene or knock-out or insertion, give it a new allele name
3) Assign a new strain name
4) Write details about how you create this strain
5) Enter into the database on wiki, give it a documentation #, which has to be in numeric order!!!
6) Write Jun an email about the update and he will proofread.
Example 1 (phenotyping based on phenotype): you created dpy-10(e128) II; wtfIs256 [myo-2p::mCherry]
Example details 1: AML283 males are crossed to CB128 dpy-10(e128). In the F2s, the plate (#2) that contains 100% pharyngeal mCherry and 100% dumpy are chosen. This new strain is named as INFxxx.
Example 2 (phenotyping based on PCR): you created eat-2(ad1113) II; zwEx104 [inx-4p::GFP + lin-15(+)]
Example details 2: HW2679 eat-2(ad1113) males are crossed to ZW284 GFP hermaphrodites. In the F2s, primer pair (xxx and xxx) are used to genotype for eat-2(ad1113). Expected size is 0.5kb. Primer xxx is used for sequencing. Plate #3 contains the zwEx104 arrays and is homozygous for eat-2(ad1113). Sequencing results can be seen at xxx. This new strain is named as INFxxx.
Documentation # | Strain Name | (geno) Type | Description | Location | Source | Notes | |||
---|---|---|---|---|---|---|---|---|---|
1 | MT17641 | nIs264[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765) | I1 marker, backcrossed 3x | Box 1, Row 1 | Horvitz (MIT) | I1, AWC, AWB expression | |||
2 | MT17912 | nIs282[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765) | on Chromosome I, backcrossed 3 times to N2 | Box 1, Row 2 | Horvitz (MIT) | I1, AWC, AWB expression | |||
3 | MT21198 | nEx1997[gpa-16 prom ::gfp; unc-54 prom ::rfp] (now gcy-10 is odr-1) | ??? paper says gpa-16 promoter but datasheet says gcy-10 | Box 1, Row 3 | Horvitz (MIT) | maintain by picking non-muv | |||
4 | MT21419 | nIs560[gcy-10 prom ::enphr3::yfp; lin-15(+)]; lin-15(n765) | YFP in AWC/I1 (faint) | Box 1, Row 4 | Horvitz (MIT) | Halorhodopsin in I1 | |||
5 | MT23561 | nIs552[gcy-10 prom ::chr2::yfp; lin-15(+)]; lite-1(ce314) gur-3(ok2245) lin-15(n765) | ChR in I1, AWC, AWB | Box 1, Row 5 | Horvitz (MIT) | used by Nikhil in paper | |||
6 | NL2334 | pkIs1273[gpa-16 prom ::gfp] | GFP in gpa-16 | Box 1, Row 6 | Horvitz (MIT) | super faint GFP in RIP | |||
7 | ZW284 | zwEx104 [inx-4p::GFP + lin-15(+)] | GFP in ADA, ADE, AIN, AUA, AVJ, DVC, FLP, PHA, PHB, PVR, PVT, RIC, RIG, RIM, RIP | Box 1, Row 7 | CGC | ||||
8 | BC11358 | pdfr-1::gfp (sIs10334 [rCesC13B9.4::GFP + pCeh361]) | Box 1, Row 8 | Horvitz (MIT) | |||||
9 | AML281 | unc-31 (DA509; AML32) | Box 1, Row 9 | unc on food, whole-brain strain | |||||
10 | AML282 | unc-31 (MT2940; AML32) | Box 2, Row 1 | unc on food, whole-brain strain | |||||
11 | PS5647 | unc-119(ed4) III; him-5(e1490) V; syIs202. | Box 2, Row 2 | syIs202 [vang-1::YFP + myo-2::DsRed + unc-119(+)]; outcrossing suggests array is integrated in LG V. Reference: Green JL, et al. Cell. 2008 Aug 22;134(4):646-56. | |||||
12 | AML283 | wtfIs256 [myo-2::mCherry] | mCherry in Pharynx, outcrossed 6x | Box 2, Row 3 | |||||
13 | AML284 | wtfIs257 [myo-2::mCherry] | mCherry in Pharynx, outcrossed 6x | Box 2, Row 4 | |||||
14 | AML327 | pha-1(e2123)III; wtfEx275 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX] | ehs-1 is supposed to be pan-pharyngel neural expression | Box 2, Row 5 | expression only in larvae | ||||
15 | AML328 | pha-1(e2123)III; wtfEx276 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX] | ehs-1 is supposed to be pan-pharyngel neural expression | Box 2, Row 6 | expression only in larvae | ||||
16 | AML32 | wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. | Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons | Box 2, Row 7 | CGC | ||||
17 | AML18 | wtfIs3 [rab-3p::NLS::GFP + rab-3p::NLS::tagRFP]. | RFP and GFP expression in the nuclei of all neurons. AML18 acts as a control for the calcium imaging strain AML14 | Box 2, Row 8 | CGC | ||||
18 | CZ23281 | juEx7105 [mec-4p::PH::miniSOG(Q103L) + mec-4p::mCherry + ttx-3::RFP] | Pick RFP+ to maintain | Box 2, Row 9 | CGC | ||||
19 | OH15262 | otIs669 V | NeuroPal | Box 3, Row 1 | CGC | ||||
20 | OH15265 | otIs672 [rab-3::NLS::GCaMP6s + arrd-4:NLS:::GCaMP6s] | Bright panneuronal nuclear GCaMP6s expression | Box 3, Row 2 | CGC | ||||
21 | OH15500 | otIs669 V; otIs672 | Box 3, Row 3 | CGC | |||||
22 | OH16230 | otIs670 V; otIs672 | Box 3, Row 4 | CGC | |||||
23 | OH16290 | otIs670 V; wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP] | Box 3, Row 5 | CGC | |||||
24 | SD1614 | ccIs4251 I; stIs10447 | ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10447 [ceh-34p::HIS-24::mCherry + unc-119(+)]. | Box 3, Row 6 | CGC | ||||
25 | ST2 | ncIs2 [pH20::GFP + pBlueScript] II | Expresses GFP in nearly all neurons | Box 3, Row 7 | CGC | ||||
26 | BR5602 | tax-4(p678) III; byEx836 [odr-4p::tax-4::GFP + myo-2p::mCherry] | Box 3, Row 8 | CGC | |||||
27 | BW1946 | ctIs43 unc-42(e270) V. | Box 3, Row 9 | CGC | |||||
28 | GRU101 | gnaIs1 [myo-2p::yfp] | Expresses YFP in pharynx | Box 4, Row 1 | CGC | ||||
29 | HA329 | lin-15B&lin-15A(n765) X; rtEx234 [nlp-13p::GFP + lin-15(+)] | Box 4, Row 2 | CGC | Raise at 20C or higher and pick non-Muv or GFP+ to maintain array. | ||||
30 | HA341 | lin-15B&lin-15A(n765) X; rtEx235 [nlp-3p::GFP + lin-15(+)]. | Box 4, Row 3 | CGC | Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain. | ||||
31 | JN1713 | peIs1713 [sra-6p::mCasp-1 + unc-122p::mCherry] | ASH neurons are eliminated. Abnormal odor chemotaxis | Box 4, Row 4 | CGC | ||||
32 | JN1715 | peIs1715 [str-1p::mCasp-1 + unc-122p::GFP]. | AWB neurons are eliminated. Abnormal odor chemotaxis | Box 4, Row 5 | CGC | ||||
33 | LP177 | unc-119(ed3) III; che-12(cp26[GFP + LoxP + unc-119(+) +LoxP]) V. | Entire che-12 coding sequence deleted and replaced with GFP. | Box 4, Row 6 | CGC | ||||
34 | LX929 | vsIs48 [unc-17::GFP]. | GFP expressed in all cholinergic neurons. | Box 4, Row 7 | CGC | ||||
35 | MH2805 | kuIs70 [alr-1p::GFP + rol-6(su1006)]. | Rollers. alr-1p::GFP expression is visible in embryonic and adult tissues. | Box 4, Row 8 | CGC | ||||
36 | MT19454 | nIs396 [sams-5 3'::4xNLS-GFP + lin-15(+)] V. | GFP expression in MI | Box 4, Row 9 | CGC | ||||
37 | NH2466 | ayIs4 I; dpy-20(e1282) IV. | Box 5, Row 1 | CGC | |||||
38 | NY2045 | ynIs45 [flp-15p::GFP] I; him-5(e1490) V. | Box 5, Row 2 | CGC | |||||
39 | NY2049 | ynIs49 [flp-5p::GFP]. | Box 5, Row 3 | CGC | |||||
40 | NY2064 | ynIs64 [flp-17p::GFP] I; him-5(e1490) V. | Box 5, Row 4 | CGC | |||||
41 | OH14372 | pha-1(e2123) III; him-5(e1490) V; otEx6713 [str-97p::GFP + pha-1(+)]. | Box 5, Row 5 | CGC | Maintain at 25C to select for transgenic array. | ||||
42 | PS3504 | syIs54 [ceh-2::GFP + unc-119(+)]; unc-119(ed4) III. | Box 5, Row 6 | CGC | Do not distribute this strain; other labs should request it from the CGC | ||||
43 | PY2417 | oyIs44 [odr-1::RFP + lin-15(+)]. | Bright RFP in AWB and AWC. | Box 5, Row 7 | CGC | ||||
44 | VM123 | lin-15B&lin-15A(n765) X; akEx29 [glr-8::GFP + lin-15(+)]. | Box 5, Row 8 | CGC | Maintain by picking non-Muv. Worms with the array are non-Muv at 20C. | ||||
45 | VM149 | lin-15B&lin-15A(n765) X; akEx38 [(pDM46) glr-7::GFP + (pJM23) lin-15(+)] | Box 5, Row 9 | CGC | Worms with the array are non-Muv at 20C. | ||||
46 | IC1204 | mIs10; him-8 | bright GFP in pharynx | Box 6, Row 1 | Ian Chin-Sang gift | ||||
47 | IC722 | mIs11; him-5 | bright GFP in pharynx | Box 6, Row 2 | Ian Chin-Sang gift | ||||
48 | IC314 | zdIs5; him-5 | GFP in 6 touch neurons | Box 6, Row 3 | Ian Chin-Sang gift | ||||
49 | INF49 | spp-12p::gfp; rol-6 as coinjection marker | GFP in NSM and I6, roller worms. | Box 6, Row 4 | Matthias Leippe | ||||
50 | ZX1917 | zxEx942[pmyo2::QuasAr::mOrange; pmyo-3::CFP] | Box 6, Row 5 | Alexander Gottschalk | |||||
51 | ZX1918 | zxEx943[pmyo-2::MacQ::mCitrine; pmyo3::mCherry] | Box 6, Row 6 | Alexander Gottschalk | |||||
52 | ZX1958 | zxEx944[pmyo-2::ArchD95N::2xmyc-tag; pmyo3::CFP] | Box 6, Row 7 | Alexander Gottschalk | |||||
53 | OK0765 | cuEx613[dbl-1 M4 enhancerwild-type] | Label M4 neuron, GFP | Box 6, Row 8 | Peter Okkema | Pick rollers | |||
54 | LSC1428 | trh-1(lst1118); lstEx776 [Pglr-8::trh-1::SL2::gfp, 50 ng/μL + pCIM02 (Punc-122::dsRED), 50 ng/μL]. | Label M4 and M5 neurons | Box 6, Row 9 | Isabel Beets | Pick GFP or RFP worms | |||
55 | MT15670 | nIs175 IV [ceh-28p::4xNLS::GFP + lin-15(+)] | M4 marker | Box 7, Row 1 | Bob Horvitz | Backcrossed 5 times | |||
56 | MT15672 | nIs177 I [ceh-28p::4NLS::GFP + lin-15(+)]. | M4 marker | Box 7, Row 2 | Bob Horvitz | Backcrossed 5 times | |||
57 | MT17913 | nIs283 X [gcy-10p::GFP + lin-15(+)] | I1 marker | Box 7, Row 3 | Bob Horvitz | Backcrossed 3 times | |||
58 | MT19378 | nIs390 III [Pflp-15∷gfp, lin-15AB(+)] | I2 marker | Box 7, Row 4 | Bob Horvitz | Backcrossed 5 times | |||
59 | MT21910 | lin-15AB(n765ts) X; nEx2065 [Pgur-3::gfp, lin-15(+)] | Expressed in I2, I4, AVD and PVC | Box 7, Row 5 | Bob Horvitz | ||||
60 | INF100 | tax-2(p671); gnaIs1 [myo-2p::yfp] | Box 7, Row 6 | Nicolina | |||||
61 | IC765 | quEx180 [rde-1p::rfp] | pick RFP to maintain | Box 7, Row 7 | Ian Chin-Sang | ||||
62 | IC2226 | quIs29 | integrated rde-1p::rfp with rgef-1p::ins-4 | Box 7, Row 8 | Ian Chin-Sang | ||||
63 | INF101 | tax-4(ok3113)III; gnaIsl[myo2-p::yfp] | YFP in pharynx, defective chemotaxis | Box 7, Row 9 | Nicolina | ||||
64 | INF102 | unc-9(e101)X; gnaIsl[myo2-p::yfp] | YFP in pharynx, unc | Box 8, Row 1 | Nicolina | ||||
65 | INF103 | pkc-1(nj1)V; gnaIsl[myo2-p::yfp] | YFP in pharynx, defective chemotaxis to AWA- and AWC- odorants | Box 8, Row 2 | Nicolina | ||||
66 | INF104 | pels1715[str-1p::mCasp-1 + unc-122p::GFP]; gnasIsl[myo2-p::yfp] | YFP in pharynx, GFP spots on underside, AWB eliminated | Box 8, Row 3 | Nicolina | Nicolina did not manage to make this strain | |||
67 | SWF62 | flvEx30[Ptph-1::GCaMP5a, Ptph-1::mCherry, Psrg-47:: GCaMP5a, Psrg-47::mCherry] | Box 8, Row 4 | Steven Flavell | |||||
68 | CX15388 | kyEx5178[tph-1(short)::HisCl1-sl2-mCherry] | Box 8, Row 5 | Steven Flavell | |||||
69 | CX15485 | unc-13(s69); lite-1(ce314); kyEx4656 [tph-1-NSMfragment::GCaMP5A; myo-3::mCherry] | Box 8, Row 6 | Steven Flavell | |||||
70 | CX16651 | kyIs694 [tph-1-NSM-fragment::rpl-22-3xHA; tph-1NSM-fragment::GFP] | Box 8, Row 7 | Steven Flavell | |||||
71 | CZ20310 | juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] unc-119(ed3) III. | optogenetic strain for integration and mutagenesis, miniSOG | Box 8, Row 8 | CGC | ||||
72 | OH15363 | otIs669 him-5(e1490) V | NeuroPal with him-5 | Box 8, Row 9 | CGC | ||||
73 | OH4141 | zdIs21 IV; wrk-1(ok695) X | Box 9, Row 1 | CGC | |||||
74 | PS6843 | syIs300 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. V | GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with transgenetic marker in next generation. | Box 9, Row 2 | CGC | Backcrossed 7 times | |||
75 | PS6844 | syIs301 [myo-2p:NLS::GAL4SC::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] V. | myo-2 cGAL driver for pharyngeal muscle. | Box 9, Row 3 | CGC | Backcrossed 5 times | |||
76 | PS6872 | syIs302 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. III. | GFP cGAL effector. | Box 9, Row 4 | CGC | Backcrossed 6 times | |||
77 | PS6963 | syIs336 [rab-3p::NLS::GAL4SK::VP64::let-858 3'UTR + unc-122p::RFP + pBlueScript]. X. | rab-3 cGAL driver for the whole nervous system. | Box 9, Row 5 | CGC | Backcrossed 4 times | |||
78 | PS7185 | syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. IV. | GFP::H2B effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. | Box 9, Row 6 | CGC | Backcrossed 3 times | |||
79 | PS7190 | syIs409 [15xUAS::?pes-10::mCherry::H2B::let-858 3'UTR + unc-122p::GFP + pBlueScript] X. | mCherry::H2B cGAL effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. | Box 9, Row 7 | CGC | Backcrossed 2 times | |||
80 | PS7199 | syIs371 [15xUAS::?pes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)] III. | Histamine chloride channel cGAL effector. | Box 9, Row 8 | CGC | Backcrossed 3 times | |||
81 | PS7200 | syIs420 [15xUAS::?pes-10::tetx::let-858 3'UTR + myo-2p::NLS::GFP + pBlueScript] IV. | Tetanus toxin cGAL effector. | Box 9, Row 9 | CGC | Backcrossed 3 times | |||
82 | PS7203 | syIs423 [15xUAS::?pes-10::GCaMP6s::SL2::mKate2::let-858 3'UTR + myo-2p::NLS::mCherry + 1kb DNA ladder(NEB)] V. | GCaMP6s cGAL effector. | Box 10, Row 1 | CGC | Backcrossed 3 times | |||
83 | INF1 | ynIs45 [flp-15p::GFP] I; otIs669 V | Box 20, Row 2 | Jun | OH15262 crossed to NY2045 | ||||
84 | INF2 | syIs54 [ceh-2::GFP + unc-119(+)] II; otIs669 V | Box 20, Row 3 | Jun | OH15262 crossed to PS3504 | ||||
85 | INF4 | odr-4 (n2144) III; gnaIs1 IV | Box 20, Row 4 | Jun | MT5300 cross to GRU101 | ||||
86 | INF5 | unc-31(e928) gnaIs1 IV | Box 20, Row 5 | Jun | DA509 cross to GRU101 | ||||
87 | JN3038 | qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100 [H20p::nls4::mCherry]; qjIs14[H20p::nls::YC2.60 | express fluorescent calcium indicator Yellow-Cameleon 2.60 with the pan-neuronal promoter H20p in the background of JN3039. Jun's note: seems to be sicker than JN3039. | Box 20, Row 6 | Gift from Yuichi Iino | ||||
88 | JN3039 | qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100 [H20p::nls4::mCherry] | roiedit3D Neuron ID strain | Box 20, Row 7 | Gift from Yuichi Iino | 21-07-20, 9 tubes | |||
89 | INF6 | nonEx1[rab-3p::his-24::tagBFP; myo-2p::mCherry::unc-54 3'utr] | To see the expression strength of rab-3p::his-24::tagBFP | Box 20, Row 8 | Jun | iLJ3 line 1 | 21-07-20, 9 tubes | ||
90 | INF7 | nonEx2[rab-3p::his-24::tagBFP; myo-2p::mCherry::unc-54 3'utr] | To see the expression strength of rab-3p::his-24::tagBFP | Box 20, Row 9 | Jun | iLJ3 line 2 | 21-07-20, 9 tubes | ||
91 | INF8 | nonEx3[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] | GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 1 | Box 21, Row 1 | Jun | iLJ4 line 1 | |||
92 | INF9 | nonEx4[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] | GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 2 | Box 21, Row 2 | Jun | iLJ4 line 2 | |||
93 | INF10 | nonEx5[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] | GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 3 | Box 21, Row 3 | Jun | iLJ4 line 3 | |||
94 | NY2030 | ynIs30 [flp-4p::GFP] I | Array seems prone to silencing. Check for GFP and pick GFP+ to maintain. | Box 21, Row 4 | CGC | ||||
95 | NY2037 | ynIs37 [flp-13p::GFP] III | Box 21, Row 5 | CGC | |||||
96 | NY2080 | ynIs80 [flp-21p::GFP]. | Box 21, Row 6 | CGC | |||||
97 | PS7401 | syIs433 IV; syIs300 V | Pmyo-2 cGAL-C driver(syIs433) | Box 21, Row 7 | Gift from Paul Sternberg | outcrossed x3 | 2020-09-09 9 tubes | ||
98 | PS7402 | syIs431 III; syIs300 V | Pmyo-2::cGAL-N (syIs431) | Box 21, Row 8 | Gift from Paul Sternberg | outcrossed x3 | 2020-09-09 9 tubes | ||
99 | PS6843 | syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL] | GFP effector | Box 21, Row 9 | Gift from Paul Sternberg | outcrossed x7 | |||
100 | PS7521 | syIs483 X [Punc-17::cGAL-N, Pceh19b:: cGAL-C] | Split cGAL drivers for MC neurons | Box 22, Row 1 | Gift from Paul Sternberg | outcrossed x3 | 2020-09-09 9 tubes | ||
101 | PS7524 | syIs371 III; syIs483 X | Split MC driver (syIs483) > HisCl1 effector (syIs371, 15xUAS::HisCl1::SL2::gfp::let-858 3’UTR) | Box 22, Row 2 | Gift from Paul Sternberg | 2020-09-09 9 tubes | |||
102 | PS7154 | syIs391 IV [myo-2p::NLS::GAL4SK::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] | myo-2 cGAL driver for pharyngeal muscle. | Box 22, Row 3 | Gift from Paul Sternberg | 2020-08-31 9 tubes | |||
103 | INF12 | syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx4[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP] | pick pan-neuronal BFP to maintain | Box 22, Row 4 | Jun | iLJ4 #2 in PS7185 background | |||
104 | INF13 | nonEx6[pPHA2∷GFP-F + Pmyo-2::mCherry::unc-54utr] | pick mCherry pharynx to maintain | Box 22, Row 5 | Jun | iLJ6 #1 | 26-08-20 9 tubes | ||
105 | INF16 | gnaIs1 IV; odr-7(ky4) X | Box 22, Row 6 | Jun | 15-09-2020 9 tubes | ||||
106 | INF17 | unc-31(n1304) gnaIs1 IV | Box 22, Row 7 | Jun | 15-09-2020 9 tubes | ||||
107 | INF18 | gnaIs1 IV; unc-9(fc16) unc-7(e5) X. | Box 22, Row 8 | Jun | |||||
108 | INF21 | syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx7[odr-1p short::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP] | odr-1p short in GAL4 driver line, directly injected in GFP effector line (PS7185) | Box 22, Row 9 | Jun | iLJ5 #1 | 10/23/2020 | ||
109 | INF22 | gnaIs1 IV; mec-4(u253) X | note: this mec-4(u253) is from Martin Chalfie's lab | Box 23, Row 1 | Jun | 2020-11-11 9 tubes | |||
110 | INF23 | gnaIs1 IV; odr-1(n1936) X. | note: it seems hard to make the cross, perhaps synthetic unhealthy | Box 23, Row 2 | Jun | 2020-11-11 9 tubes |
Numbering # | Strain Name | (geno) Type | Description | Location | Source | Notes | ||
---|---|---|---|---|---|---|---|---|
NT1 | CB4856 | Hawaiian isolate of C. elegans | Box 10, Row 1 | Grosshans (FMI), HW2675 | ||||
NT2 | N2 | wt | Box 10, Row 2 | Grosshans (FMI) | ||||
NT3 | HW2678 | eat-2(ad1116) | Box 10, Row 3 | Grosshans (FMI) | ||||
NT4 | HW2679 | eat-2(ad1113) | Box 10, Row 4 | Grosshans (FMI) | ||||
NT5 | CB101 | unc-9(e101) X | Box 10, Row 5 | CGC | ||||
NT6 | CB187 | rol-6(e187) II | Box 10, Row 6 | CGC | ||||
NT7 | CB1128 | che-7(e1128) V | Box 10, Row 7 | CGC | ||||
NT8 | DA509 | unc-31(e928) IV | Box 10, Row 8 | CGC | ||||
NT9 | MT2940 | unc-31(n1304) IV | Box 10, Row 9 | CGC | ||||
NT10 | MT6308 | eat-4(ky5) III | Box 11, Row 1 | CGC | ||||
NT11 | TQ1101 | lite-1(xu7) X | Box 11, Row 2 | CGC | ||||
NT12 | BR5514 | tax-2(p671) I; tax-4(p678) III | Box 11, Row 3 | CGC | 23-12-2019 | |||
NT13 | IK105 | pkc-1(nj1) V. | Box 11, Row 4 | CGC | 23-12-2019 | |||
NT14 | MT5300 | odr-4(n2144) III. | Box 11, Row 5 | CGC | 23-12-2019 | |||
NT15 | N2 | C. elegans wild isolate. | Box 11, Row 6 | CGC | 23-12-2019 | |||
NT16 | N2 (ancestral) | C. elegans wild type (ancestral). | Box 11, Row 7 | CGC | 23-12-2019 | |||
NT17 | PR671 | tax-2(p671) I. | Box 11, Row 8 | CGC | 23-12-2019 | |||
NT18 | PR678 | tax-4(p678) III. | Box 11, Row 9 | CGC | 23-12-2019 | |||
NT19 | PR691 | tax-2(p691) I. | Box 12, Row 1 | CGC | 23-12-2019 | |||
NT20 | VC3113 | tax-4(ok3771) III. | Box 12, Row 2 | CGC | 23-12-2019 | |||
NT21 | WM27 | rde-1(ne219) V | Box 12, Row 3 | Ian Chin-Sang | ||||
NT22 | CB5 | unc-7(e5) X. | Box 12, Row 4 | CGC | ||||
NT23 | CX4 | odr-7(ky4) X | Box 12, Row 5 | Busack, Inka | 8/5/2020 | |||
NT24 | CX2065 | odr-1(n1936) X. | Box 12, Row 6 | Busack, Inka | 8/5/2020 | |||
NT25 | CB102 | unc-10(e102) X. | Box 12, Row 7 | CGC | 8/17/2020 | |||
NT26 | CB120 | unc-4(e120) II. | Box 12, Row 8 | CGC | 8/17/2020 | |||
NT27 | CW129 | unc-9(fc16) X. | Box 12, Row 9 | CGC | 8/17/2020 | |||
NT28 | NM1657 | unc-10(md1117) X | Box 13, Row 1 | CGC | 8/17/2020 | |||
NT29 | ZM3087 | unc-9(fc16) unc-7(e5) X. | Box 13, Row 2 | CGC | 8/17/2020 | |||
NT30 | INF11 | unc-31(n1304) IV BC6 | Backcrossed 6 times | Box 13, Row 3 | Jun | 8/21/2020 9 tubes | ||
NT31 | INF14 | odr-7(ky4) X BC6 | Backcrossed 6 times | Box 13, Row 4 | Jun | 8/31/2020 | ||
NT32 | INF15 | odr-1(n1936) X. BC6 | Backcrossed 6 times | Box 13, Row 5 | Jun | 8/28/2020 | 01-09-2020 refreeze | |
NT33 | TU253 | mec-4(u253) X | Box 13, Row 6 | CGC | 9/22/2020 | |||
NT34 | INF19 | unc-10(md1117) X | BC2 | Box 13, Row 7 | Jun | |||
NT35 | INF20 | unc-10(e102) X | BC1 | Box 13, Row 8 | Jun | |||
NT36 | TU253 (MC lab) | mec-4(u253) X | maybe backcrossed | Box 13, Row 9 | Martin Chalfie | 2020-10 | ||
NT37 | TU536 | mec-10(u332) | maybe backcrossed | Box 14, Row 1 | Martin Chalfie | 10/26/2020 | ||