Worm strains

Categorized Hermaphrodite sensory receptors

https://www.wormatlas.org/hermaphrodite/nervous/Images/neurotable1leg.htm

What to do when you generate a new strain

1) Allele names must be clearly indicated.
2) If you create a new transgene or knock-out or insertion, give it a new allele name
3) Assign a new strain name
4) Write details about how you create this strain

If it comes from a genetic cross, write the strain names of the “father” and “mother”, and how you genotype them for homozygosity. If the genotyping requires PCR (or sequencing), indicate what primers you use, expected band size, and where to find the gel picture (or sequencing result).
If it comes from a transgene, write the plasmid name, co-injection marker, concentration of the plasmids, and the strain that has been injected.

5) Enter into the database on wiki, give it a documentation #, which has to be in numeric order!!!
6) Write Jun an email about the update and he will proofread.

Example 1 (phenotyping based on phenotype): you created dpy-10(e128) II; wtfIs256 [myo-2p::mCherry]
Example details 1: AML283 males are crossed to CB128 dpy-10(e128). In the F2s, the plate (#2) that contains 100% pharyngeal mCherry and 100% dumpy are chosen. This new strain is named as INFxxx.

Example 2 (phenotyping based on PCR): you created eat-2(ad1113) II; zwEx104 [inx-4p::GFP + lin-15(+)]
Example details 2: HW2679 eat-2(ad1113) males are crossed to ZW284 GFP hermaphrodites. In the F2s, primer pair (xxx and xxx) are used to genotype for eat-2(ad1113). Expected size is 0.5kb. Primer xxx is used for sequencing. Plate #3 contains the zwEx104 arrays and is homozygous for eat-2(ad1113). Sequencing results can be seen at xxx. This new strain is named as INFxxx.

Backup database for transgenic worms

Documentation #	Strain Name	(geno) Type	Description	Location	Source	Notes
1	MT17641	nIs264[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765)	I1 marker, backcrossed 3x	Box 1, Row 1	Horvitz (MIT)	I1, AWC, AWB expression
2	MT17912	nIs282[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765)	on Chromosome I, backcrossed 3 times to N2	Box 1, Row 2	Horvitz (MIT)	I1, AWC, AWB expression
3	MT21198	nEx1997[gpa-16 prom ::gfp; unc-54 prom ::rfp] (now gcy-10 is odr-1)	??? paper says gpa-16 promoter but datasheet says gcy-10	Box 1, Row 3	Horvitz (MIT)	maintain by picking non-muv
4	MT21419	nIs560[gcy-10 prom ::enphr3::yfp; lin-15(+)]; lin-15(n765)	YFP in AWC/I1 (faint)	Box 1, Row 4	Horvitz (MIT)	Halorhodopsin in I1
5	MT23561	nIs552[gcy-10 prom ::chr2::yfp; lin-15(+)]; lite-1(ce314) gur-3(ok2245) lin-15(n765)	ChR in I1, AWC, AWB	Box 1, Row 5	Horvitz (MIT)	used by Nikhil in paper
6	NL2334	pkIs1273[gpa-16 prom ::gfp]	GFP in gpa-16	Box 1, Row 6	Horvitz (MIT)	super faint GFP in RIP
7	ZW284	zwEx104 [inx-4p::GFP + lin-15(+)]	GFP in ADA, ADE, AIN, AUA, AVJ, DVC, FLP, PHA, PHB, PVR, PVT, RIC, RIG, RIM, RIP	Box 1, Row 7	CGC
8	BC11358	pdfr-1::gfp (sIs10334 [rCesC13B9.4::GFP + pCeh361])		Box 1, Row 8	Horvitz (MIT)
9	AML281	unc-31 (DA509; AML32)		Box 1, Row 9		unc on food, whole-brain strain
10	AML282	unc-31 (MT2940; AML32)		Box 2, Row 1		unc on food, whole-brain strain
11	PS5647	unc-119(ed4) III; him-5(e1490) V; syIs202.		Box 2, Row 2		syIs202 [vang-1::YFP + myo-2::DsRed + unc-119(+)]; outcrossing suggests array is integrated in LG V. Reference: Green JL, et al. Cell. 2008 Aug 22;134(4):646-56.
12	AML283	wtfIs256 [myo-2::mCherry]	mCherry in Pharynx, outcrossed 6x	Box 2, Row 3
13	AML284	wtfIs257 [myo-2::mCherry]	mCherry in Pharynx, outcrossed 6x	Box 2, Row 4
14	AML327	pha-1(e2123)III; wtfEx275 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX]	ehs-1 is supposed to be pan-pharyngel neural expression	Box 2, Row 5		expression only in larvae
15	AML328	pha-1(e2123)III; wtfEx276 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX]	ehs-1 is supposed to be pan-pharyngel neural expression	Box 2, Row 6		expression only in larvae
16	AML32	wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP].	Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons	Box 2, Row 7	CGC
17	AML18	wtfIs3 [rab-3p::NLS::GFP + rab-3p::NLS::tagRFP].	RFP and GFP expression in the nuclei of all neurons. AML18 acts as a control for the calcium imaging strain AML14	Box 2, Row 8	CGC
18	CZ23281	juEx7105 [mec-4p::PH::miniSOG(Q103L) + mec-4p::mCherry + ttx-3::RFP]	Pick RFP+ to maintain	Box 2, Row 9	CGC
19	OH15262	otIs669 V	NeuroPal	Box 3, Row 1	CGC
20	OH15265	otIs672 [rab-3::NLS::GCaMP6s + arrd-4:NLS:::GCaMP6s]	Bright panneuronal nuclear GCaMP6s expression	Box 3, Row 2	CGC
21	OH15500	otIs669 V; otIs672		Box 3, Row 3	CGC
22	OH16230	otIs670 V; otIs672		Box 3, Row 4	CGC
23	OH16290	otIs670 V; wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]		Box 3, Row 5	CGC
24	SD1614	ccIs4251 I; stIs10447	ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10447 [ceh-34p::HIS-24::mCherry + unc-119(+)].	Box 3, Row 6	CGC
25	ST2	ncIs2 [pH20::GFP + pBlueScript] II	Expresses GFP in nearly all neurons	Box 3, Row 7	CGC
26	BR5602	tax-4(p678) III; byEx836 [odr-4p::tax-4::GFP + myo-2p::mCherry]		Box 3, Row 8	CGC
27	BW1946	ctIs43 unc-42(e270) V.		Box 3, Row 9	CGC
28	GRU101	gnaIs1 [myo-2p::yfp]	Expresses YFP in pharynx	Box 4, Row 1	CGC
29	HA329	lin-15B&lin-15A(n765) X; rtEx234 [nlp-13p::GFP + lin-15(+)]		Box 4, Row 2	CGC	Raise at 20C or higher and pick non-Muv or GFP+ to maintain array.
30	HA341	lin-15B&lin-15A(n765) X; rtEx235 [nlp-3p::GFP + lin-15(+)].		Box 4, Row 3	CGC	Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain.
31	JN1713	peIs1713 [sra-6p::mCasp-1 + unc-122p::mCherry]	ASH neurons are eliminated. Abnormal odor chemotaxis	Box 4, Row 4	CGC
32	JN1715	peIs1715 [str-1p::mCasp-1 + unc-122p::GFP].	AWB neurons are eliminated. Abnormal odor chemotaxis	Box 4, Row 5	CGC
33	LP177	unc-119(ed3) III; che-12(cp26[GFP + LoxP + unc-119(+) +LoxP]) V.	Entire che-12 coding sequence deleted and replaced with GFP.	Box 4, Row 6	CGC
34	LX929	vsIs48 [unc-17::GFP].	GFP expressed in all cholinergic neurons.	Box 4, Row 7	CGC
35	MH2805	kuIs70 [alr-1p::GFP + rol-6(su1006)].	Rollers. alr-1p::GFP expression is visible in embryonic and adult tissues.	Box 4, Row 8	CGC
36	MT19454	nIs396 [sams-5 3'::4xNLS-GFP + lin-15(+)] V.	GFP expression in MI	Box 4, Row 9	CGC
37	NH2466	ayIs4 I; dpy-20(e1282) IV.		Box 5, Row 1	CGC
38	NY2045	ynIs45 [flp-15p::GFP] I; him-5(e1490) V.		Box 5, Row 2	CGC
39	NY2049	ynIs49 [flp-5p::GFP].		Box 5, Row 3	CGC
40	NY2064	ynIs64 [flp-17p::GFP] I; him-5(e1490) V.		Box 5, Row 4	CGC
41	OH14372	pha-1(e2123) III; him-5(e1490) V; otEx6713 [str-97p::GFP + pha-1(+)].		Box 5, Row 5	CGC	Maintain at 25C to select for transgenic array.
42	PS3504	syIs54 [ceh-2::GFP + unc-119(+)]; unc-119(ed4) III.		Box 5, Row 6	CGC	Do not distribute this strain; other labs should request it from the CGC
43	PY2417	oyIs44 [odr-1::RFP + lin-15(+)].	Bright RFP in AWB and AWC.	Box 5, Row 7	CGC
44	VM123	lin-15B&lin-15A(n765) X; akEx29 [glr-8::GFP + lin-15(+)].		Box 5, Row 8	CGC	Maintain by picking non-Muv. Worms with the array are non-Muv at 20C.
45	VM149	lin-15B&lin-15A(n765) X; akEx38 [(pDM46) glr-7::GFP + (pJM23) lin-15(+)]		Box 5, Row 9	CGC	Worms with the array are non-Muv at 20C.
46	IC1204	mIs10; him-8	bright GFP in pharynx	Box 6, Row 1	Ian Chin-Sang gift
47	IC722	mIs11; him-5	bright GFP in pharynx	Box 6, Row 2	Ian Chin-Sang gift
48	IC314	zdIs5; him-5	GFP in 6 touch neurons	Box 6, Row 3	Ian Chin-Sang gift
49	INF49	spp-12p::gfp; rol-6 as coinjection marker	GFP in NSM and I6, roller worms.	Box 6, Row 4	Matthias Leippe
50	ZX1917	zxEx942[pmyo2::QuasAr::mOrange; pmyo-3::CFP]		Box 6, Row 5	Alexander Gottschalk
51	ZX1918	zxEx943[pmyo-2::MacQ::mCitrine; pmyo3::mCherry]		Box 6, Row 6	Alexander Gottschalk
52	ZX1958	zxEx944[pmyo-2::ArchD95N::2xmyc-tag; pmyo3::CFP]		Box 6, Row 7	Alexander Gottschalk
53	OK0765	cuEx613[dbl-1 M4 enhancerwild-type]	Label M4 neuron, GFP	Box 6, Row 8	Peter Okkema	Pick rollers
54	LSC1428	trh-1(lst1118); lstEx776 [Pglr-8::trh-1::SL2::gfp, 50 ng/μL + pCIM02 (Punc-122::dsRED), 50 ng/μL].	Label M4 and M5 neurons	Box 6, Row 9	Isabel Beets	Pick GFP or RFP worms
55	MT15670	nIs175 IV [ceh-28p::4xNLS::GFP + lin-15(+)]	M4 marker	Box 7, Row 1	Bob Horvitz	Backcrossed 5 times
56	MT15672	nIs177 I [ceh-28p::4NLS::GFP + lin-15(+)].	M4 marker	Box 7, Row 2	Bob Horvitz	Backcrossed 5 times
57	MT17913	nIs283 X [gcy-10p::GFP + lin-15(+)]	I1 marker	Box 7, Row 3	Bob Horvitz	Backcrossed 3 times
58	MT19378	nIs390 III [Pflp-15∷gfp, lin-15AB(+)]	I2 marker	Box 7, Row 4	Bob Horvitz	Backcrossed 5 times
59	MT21910	lin-15AB(n765ts) X; nEx2065 [Pgur-3::gfp, lin-15(+)]	Expressed in I2, I4, AVD and PVC	Box 7, Row 5	Bob Horvitz
60	INF100	tax-2(p671); gnaIs1 [myo-2p::yfp]		Box 7, Row 6	Nicolina
61	IC765	quEx180 [rde-1p::rfp]	pick RFP to maintain	Box 7, Row 7	Ian Chin-Sang
62	IC2226	quIs29	integrated rde-1p::rfp with rgef-1p::ins-4	Box 7, Row 8	Ian Chin-Sang
63	INF101	tax-4(ok3113)III; gnaIsl[myo2-p::yfp]	YFP in pharynx, defective chemotaxis	Box 7, Row 9	Nicolina
64	INF102	unc-9(e101)X; gnaIsl[myo2-p::yfp]	YFP in pharynx, unc	Box 8, Row 1	Nicolina
65	INF103	pkc-1(nj1)V; gnaIsl[myo2-p::yfp]	YFP in pharynx, defective chemotaxis to AWA- and AWC- odorants	Box 8, Row 2	Nicolina
66	INF104	pels1715[str-1p::mCasp-1 + unc-122p::GFP]; gnasIsl[myo2-p::yfp]	YFP in pharynx, GFP spots on underside, AWB eliminated	Box 8, Row 3	Nicolina	Nicolina did not manage to make this strain
67	SWF62	ﬂvEx30[Ptph-1::GCaMP5a, Ptph-1::mCherry, Psrg-47:: GCaMP5a, Psrg-47::mCherry]		Box 8, Row 4	Steven Flavell
68	CX15388	kyEx5178[tph-1(short)::HisCl1-sl2-mCherry]		Box 8, Row 5	Steven Flavell
69	CX15485	unc-13(s69); lite-1(ce314); kyEx4656 [tph-1-NSMfragment::GCaMP5A; myo-3::mCherry]		Box 8, Row 6	Steven Flavell
70	CX16651	kyIs694 [tph-1-NSM-fragment::rpl-22-3xHA; tph-1NSM-fragment::GFP]		Box 8, Row 7	Steven Flavell
71	CZ20310	juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] unc-119(ed3) III.	optogenetic strain for integration and mutagenesis, miniSOG	Box 8, Row 8	CGC
72	OH15363	otIs669 him-5(e1490) V	NeuroPal with him-5	Box 8, Row 9	CGC
73	OH4141	zdIs21 IV; wrk-1(ok695) X		Box 9, Row 1	CGC
74	PS6843	syIs300 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. V	GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with transgenetic marker in next generation.	Box 9, Row 2	CGC	Backcrossed 7 times
75	PS6844	syIs301 [myo-2p:NLS::GAL4SC::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] V.	myo-2 cGAL driver for pharyngeal muscle.	Box 9, Row 3	CGC	Backcrossed 5 times
76	PS6872	syIs302 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. III.	GFP cGAL effector.	Box 9, Row 4	CGC	Backcrossed 6 times
77	PS6963	syIs336 [rab-3p::NLS::GAL4SK::VP64::let-858 3'UTR + unc-122p::RFP + pBlueScript]. X.	rab-3 cGAL driver for the whole nervous system.	Box 9, Row 5	CGC	Backcrossed 4 times
78	PS7185	syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. IV.	GFP::H2B effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.	Box 9, Row 6	CGC	Backcrossed 3 times
79	PS7190	syIs409 [15xUAS::?pes-10::mCherry::H2B::let-858 3'UTR + unc-122p::GFP + pBlueScript] X.	mCherry::H2B cGAL effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.	Box 9, Row 7	CGC	Backcrossed 2 times
80	PS7199	syIs371 [15xUAS::?pes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)] III.	Histamine chloride channel cGAL effector.	Box 9, Row 8	CGC	Backcrossed 3 times
81	PS7200	syIs420 [15xUAS::?pes-10::tetx::let-858 3'UTR + myo-2p::NLS::GFP + pBlueScript] IV.	Tetanus toxin cGAL effector.	Box 9, Row 9	CGC	Backcrossed 3 times
82	PS7203	syIs423 [15xUAS::?pes-10::GCaMP6s::SL2::mKate2::let-858 3'UTR + myo-2p::NLS::mCherry + 1kb DNA ladder(NEB)] V.	GCaMP6s cGAL effector.	Box 10, Row 1	CGC	Backcrossed 3 times
83	INF1	ynIs45 [flp-15p::GFP] I; otIs669 V		Box 20, Row 2	Jun	OH15262 crossed to NY2045
84	INF2	syIs54 [ceh-2::GFP + unc-119(+)] II; otIs669 V		Box 20, Row 3	Jun	OH15262 crossed to PS3504
85	INF4	odr-4 (n2144) III; gnaIs1 IV		Box 20, Row 4	Jun	MT5300 cross to GRU101
86	INF5	unc-31(e928) gnaIs1 IV		Box 20, Row 5	Jun	DA509 cross to GRU101
87	JN3038	qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100 [H20p::nls4::mCherry]; qjIs14[H20p::nls::YC2.60	express fluorescent calcium indicator Yellow-Cameleon 2.60 with the pan-neuronal promoter H20p in the background of JN3039. Jun's note: seems to be sicker than JN3039.	Box 20, Row 6	Gift from Yuichi Iino
88	JN3039	qjIs11[glr-1p::svnls2::TagBFPsyn, ser-2(prom2)p::svnls2::TagBFPsyn]; peIs3042[eat-4p::svnls2::TagRFP675syn, lin-44p::GFP]; peIs2100 [H20p::nls4::mCherry]	roiedit3D Neuron ID strain	Box 20, Row 7	Gift from Yuichi Iino		21-07-20, 9 tubes
89	INF6	nonEx1[rab-3p::his-24::tagBFP; myo-2p::mCherry::unc-54 3'utr]	To see the expression strength of rab-3p::his-24::tagBFP	Box 20, Row 8	Jun	iLJ3 line 1	21-07-20, 9 tubes
90	INF7	nonEx2[rab-3p::his-24::tagBFP; myo-2p::mCherry::unc-54 3'utr]	To see the expression strength of rab-3p::his-24::tagBFP	Box 20, Row 9	Jun	iLJ3 line 2	21-07-20, 9 tubes
91	INF8	nonEx3[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP]	GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 1	Box 21, Row 1	Jun	iLJ4 line 1
92	INF9	nonEx4[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP]	GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 2	Box 21, Row 2	Jun	iLJ4 line 2
93	INF10	nonEx5[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP]	GAL4 driver line, with odr-1p 1.7kb (I1, AWB, AWC), line 3	Box 21, Row 3	Jun	iLJ4 line 3
94	NY2030	ynIs30 [flp-4p::GFP] I	Array seems prone to silencing. Check for GFP and pick GFP+ to maintain.	Box 21, Row 4	CGC
95	NY2037	ynIs37 [flp-13p::GFP] III		Box 21, Row 5	CGC
96	NY2080	ynIs80 [flp-21p::GFP].		Box 21, Row 6	CGC
97	PS7401	syIs433 IV; syIs300 V	Pmyo-2 cGAL-C driver(syIs433)	Box 21, Row 7	Gift from Paul Sternberg	outcrossed x3	2020-09-09 9 tubes
98	PS7402	syIs431 III; syIs300 V	Pmyo-2::cGAL-N (syIs431)	Box 21, Row 8	Gift from Paul Sternberg	outcrossed x3	2020-09-09 9 tubes
99	PS6843	syIs300 V [pG4US7(15xUAS::∆pes-10::gfp::unc-54 3'UTR), 25 ng/µL; Pttx3::rfp, 40 ng/µL; pBlueScript, 35 ng/µL]	GFP effector	Box 21, Row 9	Gift from Paul Sternberg	outcrossed x7
100	PS7521	syIs483 X [Punc-17::cGAL-N, Pceh19b:: cGAL-C]	Split cGAL drivers for MC neurons	Box 22, Row 1	Gift from Paul Sternberg	outcrossed x3	2020-09-09 9 tubes
101	PS7524	syIs371 III; syIs483 X	Split MC driver (syIs483) > HisCl1 effector (syIs371, 15xUAS::HisCl1::SL2::gfp::let-858 3’UTR)	Box 22, Row 2	Gift from Paul Sternberg		2020-09-09 9 tubes
102	PS7154	syIs391 IV [myo-2p::NLS::GAL4SK::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)]	myo-2 cGAL driver for pharyngeal muscle.	Box 22, Row 3	Gift from Paul Sternberg		2020-08-31 9 tubes
103	INF12	syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx4[odr-1p 1.7kb::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR; rab-3p::his-24::tagBFP]	pick pan-neuronal BFP to maintain	Box 22, Row 4	Jun	iLJ4 #2 in PS7185 background
104	INF13	nonEx6[pPHA2∷GFP-F + Pmyo-2::mCherry::unc-54utr]	pick mCherry pharynx to maintain	Box 22, Row 5	Jun	iLJ6 #1	26-08-20 9 tubes
105	INF16	gnaIs1 IV; odr-7(ky4) X		Box 22, Row 6	Jun		15-09-2020 9 tubes
106	INF17	unc-31(n1304) gnaIs1 IV		Box 22, Row 7	Jun		15-09-2020 9 tubes
107	INF18	gnaIs1 IV; unc-9(fc16) unc-7(e5) X.		Box 22, Row 8	Jun
108	INF21	syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript] IV; nonEx7[odr-1p short::NLS::cGAL(DBD):: cGAL(AD)::let-858 3′UTR + rab-3p-his-24::tagBFP]	odr-1p short in GAL4 driver line, directly injected in GFP effector line (PS7185)	Box 22, Row 9	Jun	iLJ5 #1	10/23/2020
109	INF22	gnaIs1 IV; mec-4(u253) X	note: this mec-4(u253) is from Martin Chalfie's lab	Box 23, Row 1	Jun		2020-11-11 9 tubes
110	INF23	gnaIs1 IV; odr-1(n1936) X.	note: it seems hard to make the cross, perhaps synthetic unhealthy	Box 23, Row 2	Jun		2020-11-11 9 tubes

Backup mutant database (ie. non-transgenics)

Numbering #	Strain Name	(geno) Type	Description	Location	Source	Notes
NT1	CB4856	Hawaiian isolate of C. elegans		Box 10, Row 1	Grosshans (FMI), HW2675
NT2	N2	wt		Box 10, Row 2	Grosshans (FMI)
NT3	HW2678	eat-2(ad1116)		Box 10, Row 3	Grosshans (FMI)
NT4	HW2679	eat-2(ad1113)		Box 10, Row 4	Grosshans (FMI)
NT5	CB101	unc-9(e101) X		Box 10, Row 5	CGC
NT6	CB187	rol-6(e187) II		Box 10, Row 6	CGC
NT7	CB1128	che-7(e1128) V		Box 10, Row 7	CGC
NT8	DA509	unc-31(e928) IV		Box 10, Row 8	CGC
NT9	MT2940	unc-31(n1304) IV		Box 10, Row 9	CGC
NT10	MT6308	eat-4(ky5) III		Box 11, Row 1	CGC
NT11	TQ1101	lite-1(xu7) X		Box 11, Row 2	CGC
NT12	BR5514	tax-2(p671) I; tax-4(p678) III		Box 11, Row 3	CGC	23-12-2019
NT13	IK105	pkc-1(nj1) V.		Box 11, Row 4	CGC	23-12-2019
NT14	MT5300	odr-4(n2144) III.		Box 11, Row 5	CGC	23-12-2019
NT15	N2	C. elegans wild isolate.		Box 11, Row 6	CGC	23-12-2019
NT16	N2 (ancestral)	C. elegans wild type (ancestral).		Box 11, Row 7	CGC	23-12-2019
NT17	PR671	tax-2(p671) I.		Box 11, Row 8	CGC	23-12-2019
NT18	PR678	tax-4(p678) III.		Box 11, Row 9	CGC	23-12-2019
NT19	PR691	tax-2(p691) I.		Box 12, Row 1	CGC	23-12-2019
NT20	VC3113	tax-4(ok3771) III.		Box 12, Row 2	CGC	23-12-2019
NT21	WM27	rde-1(ne219) V		Box 12, Row 3	Ian Chin-Sang
NT22	CB5	unc-7(e5) X.		Box 12, Row 4	CGC
NT23	CX4	odr-7(ky4) X		Box 12, Row 5	Busack, Inka	8/5/2020
NT24	CX2065	odr-1(n1936) X.		Box 12, Row 6	Busack, Inka	8/5/2020
NT25	CB102	unc-10(e102) X.		Box 12, Row 7	CGC	8/17/2020
NT26	CB120	unc-4(e120) II.		Box 12, Row 8	CGC	8/17/2020
NT27	CW129	unc-9(fc16) X.		Box 12, Row 9	CGC	8/17/2020
NT28	NM1657	unc-10(md1117) X		Box 13, Row 1	CGC	8/17/2020
NT29	ZM3087	unc-9(fc16) unc-7(e5) X.		Box 13, Row 2	CGC	8/17/2020
NT30	INF11	unc-31(n1304) IV BC6	Backcrossed 6 times	Box 13, Row 3	Jun	8/21/2020 9 tubes
NT31	INF14	odr-7(ky4) X BC6	Backcrossed 6 times	Box 13, Row 4	Jun	8/31/2020
NT32	INF15	odr-1(n1936) X. BC6	Backcrossed 6 times	Box 13, Row 5	Jun	8/28/2020	01-09-2020 refreeze
NT33	TU253	mec-4(u253) X		Box 13, Row 6	CGC	9/22/2020
NT34	INF19	unc-10(md1117) X	BC2	Box 13, Row 7	Jun
NT35	INF20	unc-10(e102) X	BC1	Box 13, Row 8	Jun
NT36	TU253 (MC lab)	mec-4(u253) X	maybe backcrossed	Box 13, Row 9	Martin Chalfie	2020-10
NT37	TU536	mec-10(u332)	maybe backcrossed	Box 14, Row 1	Martin Chalfie	10/26/2020

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