current database

https://www.wormatlas.org/hermaphrodite/nervous/Images/neurotable1leg.htm

1) Allele names must be clearly indicated.
2) If you create a new transgene or knock-out or insertion, give it a new allele name
3) Assign a new strain name
4) Write details about how you create this strain

• If it comes from a genetic cross, write the strain names of the “father” and “mother”, and how you genotype them for homozygosity. If the genotyping requires PCR (or sequencing), indicate what primers you use, expected band size, and where to find the gel picture (or sequencing result).
  
• If it comes from a transgene, write the plasmid name, co-injection marker, concentration of the plasmids, and the strain that has been injected.
  

5) Enter into the database on wiki, give it a documentation #, which has to be in numeric order!!!
6) Write Jun an email about the update and he will proofread.

Example 1 (phenotyping based on phenotype): you created dpy-10(e128) II; wtfIs256 [myo-2p::mCherry]
Example details 1: AML283 males are crossed to CB128 dpy-10(e128). In the F2s, the plate (#2) that contains 100% pharyngeal mCherry and 100% dumpy are chosen. This new strain is named as INFxxx.

Example 2 (phenotyping based on PCR): you created eat-2(ad1113) II; zwEx104 [inx-4p::GFP + lin-15(+)]
Example details 2: HW2679 eat-2(ad1113) males are crossed to ZW284 GFP hermaphrodites. In the F2s, primer pair (xxx and xxx) are used to genotype for eat-2(ad1113). Expected size is 0.5kb. Primer xxx is used for sequencing. Plate #3 contains the zwEx104 arrays and is homozygous for eat-2(ad1113). Sequencing results can be seen at xxx. This new strain is named as INFxxx.