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Worm strains

Categorized Hermaphrodite sensory receptors

What to do when you generate a new strain

1) Allele names must be clearly indicated.
2) If you create a new transgene or knock-out or insertion, give it a new allele name
3) Assign a new strain name
4) Write details about how you create this strain

  • If it comes from a genetic cross, write the strain names of the “father” and “mother”, and how you genotype them for homozygosity. If the genotyping requires PCR (or sequencing), indicate what primers you use, expected band size, and where to find the gel picture (or sequencing result).
  • If it comes from a transgene, write the plasmid name, co-injection marker, concentration of the plasmids, and the strain that has been injected.

5) Enter into the database on wiki, give it a documentation #, which has to be in numeric order!!!
6) Write Jun an email about the update and he will proofread.

Example 1 (phenotyping based on phenotype): you created dpy-10(e128) II; wtfIs256 [myo-2p::mCherry]
Example details 1: AML283 males are crossed to CB128 dpy-10(e128). In the F2s, the plate (#2) that contains 100% pharyngeal mCherry and 100% dumpy are chosen. This new strain is named as INFxxx.

Example 2 (phenotyping based on PCR): you created eat-2(ad1113) II; zwEx104 [inx-4p::GFP + lin-15(+)]
Example details 2: HW2679 eat-2(ad1113) males are crossed to ZW284 GFP hermaphrodites. In the F2s, primer pair (xxx and xxx) are used to genotype for eat-2(ad1113). Expected size is 0.5kb. Primer xxx is used for sequencing. Plate #3 contains the zwEx104 arrays and is homozygous for eat-2(ad1113). Sequencing results can be seen at xxx. This new strain is named as INFxxx.

Backup database for transgenic worms

Documentation # Strain Name (geno) Type Description Location Source Notes
1 MT17641 nIs264[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765) I1 marker, backcrossed 3x Box 1, Row 1 Horvitz (MIT) I1, AWC, AWB expression
2 MT17912 nIs282[gcy-10 prom ::4xNLS::gfp, lin-15(+)]; lin-15(n765) on Chromosome I, backcrossed 3 times to N2 Box 1, Row 2 Horvitz (MIT) I1, AWC, AWB expression
3 MT21198 nEx1997[gpa-16 prom ::gfp; unc-54 prom ::rfp] (now gcy-10 is odr-1) ??? paper says gpa-16 promoter but datasheet says gcy-10 Box 1, Row 3 Horvitz (MIT) maintain by picking non-muv
4 MT21419 nIs560[gcy-10 prom ::enphr3::yfp; lin-15(+)]; lin-15(n765) YFP in AWC/I1 (faint) Box 1, Row 4 Horvitz (MIT) Halorhodopsin in I1
5 MT23561 nIs552[gcy-10 prom ::chr2::yfp; lin-15(+)]; lite-1(ce314) gur-3(ok2245) lin-15(n765) ChR in I1, AWC, AWB Box 1, Row 5 Horvitz (MIT) used by Nikhil in paper
6 NL2334 pkIs1273[gpa-16 prom ::gfp] GFP in gpa-16 Box 1, Row 6 Horvitz (MIT) super faint GFP in RIP
7 ZW284 zwEx104 [inx-4p::GFP + lin-15(+)] GFP in ADA, ADE, AIN, AUA,
Box 1, Row 7 CGC
8 BC11358 pdfr-1::gfp (sIs10334 [rCesC13B9.4::GFP + pCeh361]) Box 1, Row 8 Horvitz (MIT)
9 AML281 unc-31 (DA509; AML32) Box 1, Row 9 unc on food, whole-brain strain
10 AML282 unc-31 (MT2940; AML32) Box 2, Row 1 unc on food, whole-brain strain
11 PS5647 unc-119(ed4) III; him-5(e1490) V; syIs202. Box 2, Row 2 syIs202 [vang-1::YFP + myo-2::DsRed + unc-119(+)]; outcrossing suggests array is integrated in LG V. Reference: Green JL, et al. Cell. 2008 Aug 22;134(4):646-56.
12 AML283 wtfIs256 [myo-2::mCherry] mCherry in Pharynx, outcrossed 6x Box 2, Row 3
13 AML284 wtfIs257 [myo-2::mCherry] mCherry in Pharynx, outcrossed 6x Box 2, Row 4
14 AML327 pha-1(e2123)III; wtfEx275 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX] ehs-1 is supposed to be pan-pharyngel neural expression Box 2, Row 5 expression only in larvae
15 AML328 pha-1(e2123)III; wtfEx276 [j20-ehs-1-“62bps”::his-24::tagBFP::unc-54 + pBX] ehs-1 is supposed to be pan-pharyngel neural expression Box 2, Row 6 expression only in larvae
16 AML32 wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons Box 2, Row 7 CGC
17 AML18 wtfIs3 [rab-3p::NLS::GFP + rab-3p::NLS::tagRFP]. RFP and GFP expression in the nuclei of all neurons. AML18 acts as a control for the calcium imaging strain AML14 Box 2, Row 8 CGC
18 CZ23281 juEx7105 [mec-4p::PH::miniSOG(Q103L) + mec-4p::mCherry + ttx-3::RFP] Pick RFP+ to maintain Box 2, Row 9 CGC
19 OH15262 otIs669 V NeuroPal Box 3, Row 1 CGC
20 OH15265 otIs672 [rab-3::NLS::GCaMP6s + arrd-4:NLS:::GCaMP6s] Bright panneuronal nuclear GCaMP6s expression Box 3, Row 2 CGC
21 OH15500 otIs669 V; otIs672 Box 3, Row 3 CGC
22 OH16230 otIs670 V; otIs672 Box 3, Row 4 CGC
23 OH16290 otIs670 V; wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP] Box 3, Row 5 CGC
24 SD1614 ccIs4251 I; stIs10447 ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10447 [ceh-34p::HIS-24::mCherry + unc-119(+)]. Box 3, Row 6 CGC
25 ST2 ncIs2 [pH20::GFP + pBlueScript] II Expresses GFP in nearly all neurons Box 3, Row 7 CGC
26 BR5602 tax-4(p678) III; byEx836 [odr-4p::tax-4::GFP + myo-2p::mCherry] Box 3, Row 8 CGC
27 BW1946 ctIs43 unc-42(e270) V. Box 3, Row 9 CGC
28 GRU101 gnaIs1 [myo-2p::yfp] Expresses YFP in pharynx Box 4, Row 1 CGC
29 HA329 lin-15B&lin-15A(n765) X; rtEx234 [nlp-13p::GFP + lin-15(+)] Box 4, Row 2 CGC Raise at 20C or higher and pick non-Muv or GFP+ to maintain array.
30 HA341 lin-15B&lin-15A(n765) X; rtEx235 [nlp-3p::GFP + lin-15(+)]. Box 4, Row 3 CGC Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain.
31 JN1713 peIs1713 [sra-6p::mCasp-1 + unc-122p::mCherry] ASH neurons are eliminated. Abnormal odor chemotaxis Box 4, Row 4 CGC
32 JN1715 peIs1715 [str-1p::mCasp-1 + unc-122p::GFP]. AWB neurons are eliminated. Abnormal odor chemotaxis Box 4, Row 5 CGC
33 LP177 unc-119(ed3) III; che-12(cp26[GFP + LoxP + unc-119(+) +LoxP]) V. Entire che-12 coding sequence deleted and replaced with GFP. Box 4, Row 6 CGC
34 LX929  vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. Box 4, Row 7 CGC
35 MH2805  kuIs70 [alr-1p::GFP + rol-6(su1006)]. Rollers. alr-1p::GFP expression is visible in embryonic and adult tissues. Box 4, Row 8 CGC
36 MT19454 nIs396 [sams-5 3'::4xNLS-GFP + lin-15(+)] V.  GFP expression in MI Box 4, Row 9 CGC
37 NH2466  ayIs4 I; dpy-20(e1282) IV.  Box 5, Row 1 CGC
38 NY2045 ynIs45 [flp-15p::GFP] I; him-5(e1490) V. Box 5, Row 2 CGC
39 NY2049 ynIs49 [flp-5p::GFP]. Box 5, Row 3 CGC
40 NY2064  ynIs64 [flp-17p::GFP] I; him-5(e1490) V. Box 5, Row 4 CGC
41 OH14372 pha-1(e2123) III; him-5(e1490) V; otEx6713 [str-97p::GFP + pha-1(+)]. Box 5, Row 5 CGC Maintain at 25C to select for transgenic array. 
42 PS3504  syIs54 [ceh-2::GFP + unc-119(+)]; unc-119(ed4) III. Box 5, Row 6 CGC Do not distribute this strain; other labs should request it from the CGC
43 PY2417 oyIs44 [odr-1::RFP + lin-15(+)]. Bright RFP in AWB and AWC. Box 5, Row 7 CGC
44 VM123 lin-15B&lin-15A(n765) X; akEx29 [glr-8::GFP + lin-15(+)]. Box 5, Row 8 CGC Maintain by picking non-Muv. Worms with the array are non-Muv at 20C.
45 VM149 lin-15B&lin-15A(n765) X; akEx38 [(pDM46) glr-7::GFP + (pJM23) lin-15(+)] Box 5, Row 9 CGC Worms with the array are non-Muv at 20C.
46 IC1204 mIs10; him-8 bright GFP in pharynx Box 6, Row 1 Ian Chin-Sang gift
47 IC722 mIs11; him-5 bright GFP in pharynx Box 6, Row 2 Ian Chin-Sang gift
48 IC314 zdIs5; him-5 GFP in 6 touch neurons Box 6, Row 3 Ian Chin-Sang gift
49 INF49 spp-12p::gfp; rol-6 as coinjection marker GFP in NSM and I6, roller worms. Box 6, Row 4 Matthias Leippe
50 ZX1917 zxEx942[pmyo2::QuasAr::mOrange; pmyo-3::CFP] Box 6, Row 5 Alexander Gottschalk
51 ZX1918 zxEx943[pmyo-2::MacQ::mCitrine; pmyo3::mCherry] Box 6, Row 6 Alexander Gottschalk
52 ZX1958 zxEx944[pmyo-2::ArchD95N::2xmyc-tag; pmyo3::CFP] Box 6, Row 7 Alexander Gottschalk
53 OK0765 cuEx613[dbl-1 M4 enhancerwild-type] Label M4 neuron, GFP Box 6, Row 8 Peter Okkema Pick rollers
54 LSC1428 trh-1(lst1118); lstEx776 [Pglr-8::trh-1::SL2::gfp, 50 ng/μL + pCIM02 (Punc-122::dsRED), 50 ng/μL]. Label M4 and M5 neurons Box 6, Row 9 Isabel Beets Pick GFP or RFP worms
55 MT15670 nIs175 IV [ceh-28p::4xNLS::GFP + lin-15(+)] M4 marker Box 7, Row 1 Bob Horvitz Backcrossed 5 times
56 MT15672 nIs177 I [ceh-28p::4NLS::GFP + lin-15(+)]. M4 marker Box 7, Row 2 Bob Horvitz Backcrossed 5 times
57 MT17913 nIs283 X [gcy-10p::GFP + lin-15(+)] I1 marker Box 7, Row 3 Bob Horvitz Backcrossed 3 times
58 MT19378 nIs390 III [Pflp-15∷gfp, lin-15AB(+)] I2 marker Box 7, Row 4 Bob Horvitz Backcrossed 5 times
59 MT21910 lin-15AB(n765ts) X; nEx2065 [Pgur-3::gfp, lin-15(+)] Expressed in I2, I4, AVD and PVC Box 7, Row 5 Bob Horvitz
60 INF100 tax-2(p671); gnaIs1 [myo-2p::yfp] Box 7, Row 6 Nicolina
61 IC765 quEx180 [rde-1p::rfp] pick RFP to maintain Box 7, Row 7 Ian Chin-Sang
62 IC2226 quIs29 integrated rde-1p::rfp with rgef-1p::ins-4 Box 7, Row 8 Ian Chin-Sang
63 INF101 tax-4(ok3113)III; gnaIsl[myo2-p::yfp] YFP in pharynx, defective chemotaxis Box 7, Row 9 Nicolina
64 INF102 unc-9(e101)X; gnaIsl[myo2-p::yfp] YFP in pharynx, unc Box 8, Row 1 Nicolina
65 INF103 pkc-1(nj1)V; gnaIsl[myo2-p::yfp] YFP in pharynx, defective chemotaxis to AWA- and AWC- odorants Box 8, Row 2 Nicolina
66 INF104 pels1715[str-1p::mCasp-1 + unc-122p::GFP]; gnasIsl[myo2-p::yfp] YFP in pharynx, GFP spots on underside, AWB eliminated Box 8, Row 3 Nicolina
67 SWF62 flvEx30[Ptph-1::GCaMP5a, Ptph-1::mCherry, Psrg-47:: GCaMP5a, Psrg-47::mCherry] Box 8, Row 4 Steven Flavell
68 CX15388 kyEx5178[tph-1(short)::HisCl1-sl2-mCherry] Box 8, Row 5 Steven Flavell
69 CX15485 unc-13(s69); lite-1(ce314); kyEx4656 [tph-1-NSMfragment::GCaMP5A; myo-3::mCherry] Box 8, Row 6 Steven Flavell
70 CX16651 kyIs694 [tph-1-NSM-fragment::rpl-22-3xHA; tph-1NSM-fragment::GFP] Box 8, Row 7 Steven Flavell
71 CZ20310 juSi164 unc-119(ed3) III. optogenetic strain for integration and mutagenesis Box 8, Row 8 CGC
72 OH15363 otIs669 him-5(e1490) V NeuroPal with him-5 Box 8, Row 9 CGC
73 OH4141 zdIs21 IV; wrk-1(ok695) X Box 9, Row 1 CGC
74 PS6843 syIs300 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript].  V GFP cGAL effector.  Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with transgenetic marker in next generation.  Box 9, Row 2 CGC Backcrossed 7 times
75 PS6844 syIs301 [myo-2p:NLS::GAL4SC::VP64::unc-54 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] V. myo-2 cGAL driver for pharyngeal muscle. Box 9, Row 3 CGC Backcrossed 5 times
76 PS6872 syIs302 [15xUAS::?pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. III. GFP cGAL effector.  Box 9, Row 4 CGC Backcrossed 6 times
77 PS6963 syIs336 [rab-3p::NLS::GAL4SK::VP64::let-858 3'UTR + unc-122p::RFP +  pBlueScript]. X. rab-3 cGAL driver for the whole nervous system.  Box 9, Row 5 CGC Backcrossed 4 times
78 PS7185 syIs406 [15xUAS::?pes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. IV. GFP::H2B effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.  Box 9, Row 6 CGC Backcrossed 3 times
79 PS7190 syIs409 [15xUAS::?pes-10::mCherry::H2B::let-858 3'UTR + unc-122p::GFP + pBlueScript] X. mCherry::H2B cGAL effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. Box 9, Row 7 CGC Backcrossed 2 times
80 PS7199 syIs371 [15xUAS::?pes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)] III. Histamine chloride channel cGAL effector. Box 9, Row 8 CGC Backcrossed 3 times
81 PS7200 syIs420 [15xUAS::?pes-10::tetx::let-858 3'UTR + myo-2p::NLS::GFP + pBlueScript] IV. Tetanus toxin cGAL effector. Box 9, Row 9 CGC Backcrossed 3 times
82 PS7203 syIs423 [15xUAS::?pes-10::GCaMP6s::SL2::mKate2::let-858 3'UTR + myo-2p::NLS::mCherry + 1kb DNA ladder(NEB)] V. GCaMP6s cGAL effector. Box 10, Row 1 CGC Backcrossed 3 times

Backup mutant database (ie. non-transgenics)

Numbering # Strain Name (geno) Type Description Location Source Notes
NT1 CB4856 Hawaiian isolate of C. elegans Box 10, Row 1 Grosshans (FMI), HW2675
NT2 N2 wt Box 10, Row 2 Grosshans (FMI)
NT3 HW2678 eat-2(ad1116) Box 10, Row 3 Grosshans (FMI)
NT4 HW2679 eat-2(ad1113) Box 10, Row 4 Grosshans (FMI)
NT5 CB101 unc-9(e101) X Box 10, Row 5 CGC
NT6 CB187 rol-6(e187) II Box 10, Row 6 CGC
NT7 CB1128 che-7(e1128) V Box 10, Row 7 CGC
NT8 DA509 unc-31(e928) IV Box 10, Row 8 CGC
NT9 MT2940 unc-31(n1304) IV Box 10, Row 9 CGC
NT10 MT6308 eat-4(ky5) III Box 11, Row 1 CGC
NT11 TQ1101 lite-1(xu7) X Box 11, Row 2 CGC
NT12 BR5514  tax-2(p671) I; tax-4(p678) III Box 11, Row 3 CGC 23-12-2019
NT13 IK105 pkc-1(nj1) V. Box 11, Row 4 CGC 23-12-2019
NT14 MT5300 odr-4(n2144) III. Box 11, Row 5 CGC 23-12-2019
NT15 N2 C. elegans wild isolate. Box 11, Row 6 CGC 23-12-2019
NT16 N2 (ancestral) C. elegans wild type (ancestral). Box 11, Row 7 CGC 23-12-2019
NT17 PR671  tax-2(p671) I. Box 11, Row 8 CGC 23-12-2019
NT18 PR678 tax-4(p678) III. Box 11, Row 9 CGC 23-12-2019
NT19 PR691 tax-2(p691) I. Box 12, Row 1 CGC 23-12-2019
NT20 VC3113 tax-4(ok3771) III. Box 12, Row 2 CGC 23-12-2019
NT21 WM27 rde-1(ne219) V Box 12, Row 3 Ian Chin-Sang
NT22 CB5 unc-7(e5) X. Box 12, Row 4 CGC
wiki/documentation/strains.txt · Last modified: 2020/03/23 17:02 by jliu