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wiki:documentation:wiki:documentation:jun:11-05-2020_clone_odr-1p_ceh-34p_into_gal4_vector_using_new_primers [2020/05/13 13:42]
jliu created
wiki:documentation:wiki:documentation:jun:11-05-2020_clone_odr-1p_ceh-34p_into_gal4_vector_using_new_primers [2020/05/13 13:46] (current)
jliu
Line 67: Line 67:
 pHW393/F+A is very weak. But still usable. ​ \\  ​ pHW393/F+A is very weak. But still usable. ​ \\  ​
  
 +
 +Step III: To ligate \\ 
 +
 +Lig A \\ 
 +pHW393/F+A: 10 \\ 
 +27/28/F+A: 3 \\ 
 +10X Fast ligation buffer: 2 \\ 
 +T4: 1 \\ 
 +H2O: 4 \\ 
 +
 +Lig B \\ 
 +pHW393/F+A: 10 \\ 
 +27/29/F+A: 2 \\ 
 +10X Fast ligation buffer: 2 \\ 
 +T4: 1 \\ 
 +H2O: 5 \\ 
 +
 +Lig C \\ 
 +pHW393/F+A: 10 \\ 
 +25/26/F+A: 7 \\ 
 +10X Fast ligation buffer: 2 \\ 
 +T4: 1 \\ 
 +
 +
 +22C, 10min
  
  
  
wiki/documentation/wiki/documentation/jun/11-05-2020_clone_odr-1p_ceh-34p_into_gal4_vector_using_new_primers.txt ยท Last modified: 2020/05/13 13:46 by jliu

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