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wiki:documentation:wiki:documentation:natascha:autofocus_testing_with_worms [2020/06/18 10:01] (current)
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|+||====== Autofocus testing with worms ======|
|+||* Autofocus function itself works (choses right frame within recorded images), but when reopening pylonViewer the worm is out of focus|
|+||* When running the function multiple times without moving the sample, half the time the focus is correctly identified in frame 15, the other half it seems like the focus is correctly identified in frame 16 but when opening pylon the worm is only actually in focus for focalplane 15|
|+||* Testing a batch of different lawns showed that the focalplane was always around frame 15(±1), so I will make the range over which images are taken smaller and decrease stepsize to see whether this solves the issue|