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wiki:documentation:wiki:documentation:nicolina:10.12.19 [2019/12/11 09:59]
nzjacic created
wiki:documentation:wiki:documentation:nicolina:10.12.19 [2019/12/11 10:07] (current)
nzjacic added OP50 ON
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   * Finish 0006 manual data collection   * Finish 0006 manual data collection
   * Sync AML-283, AML-284 and N2   * Sync AML-283, AML-284 and N2
 +  * Set up O/N of OP50-GFP and streak onto LB-Amp plate
  
 ===== Observations ===== ===== Observations =====
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     * AML-284 laid a surprising amount of eggs rivaling AML-283 when usually egg-laying is weaker     * AML-284 laid a surprising amount of eggs rivaling AML-283 when usually egg-laying is weaker
     * AML-283 surprisingly either on par with or less than AML-284 - will see what proportion hatch tomorrow     * AML-283 surprisingly either on par with or less than AML-284 - will see what proportion hatch tomorrow
 +  * OP50-GFP O/N - 20ul Amp into 20ml LB for 1/1000 dilution¬†
 +  * OP50-GFP streaking - 80ul LB + 20ul Amp spread onto plate - let dry for 10 min
  
wiki/documentation/wiki/documentation/nicolina/10.12.19.txt · Last modified: 2019/12/11 10:07 by nzjacic

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