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wiki:documentation:wiki:documentation:nicolina:10.2.20 [2020/02/10 07:13] (current)
nzjacic created
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 +====== 10.2.20 ======
 +
 +===== Objectives =====
 +
 +  * Check IK105 chunk
 +  * Maintain all strains and crosses
 +  * Check F2 of tax-2/4 crosses –> genotyping plates
 +  * Create primers for IK105 and JN1715
 +    * Check potential of GFP as a marker for JN1715
 +  * Sync GRU101
 +  * Make Amp plate (20ul amp + 80ul LB --> L-spread) and let dry
 +
 +===== Observations =====
 +
 +  * JN1715 shouldn't need a primer because of unc-122p::GFP
 +    * GFP signal bleeds through to YFP filter but should be fine
 +    * Signal looks like little spots on the sides of the worm - different to pharyngeal GRU101 signal
 +  * IK105 - unfortunately has an enzyme cutter when mutation in pkc-1(nj1) is present but too unspecific - has to be sequenced for genotyping instead - put F and R primer in Dropbox for Jun
 +  * IK105 chunk had multiple adults on it instead of just 1 or 2 - bleached JN1715 doesn't have any other contamination other than what was already on the plate (been experiencing an unusual type of contamination within the agar since last week - we're currently trying to troubleshoot the problem)
 +
  
wiki/documentation/wiki/documentation/nicolina/10.2.20.txt · Last modified: 2020/02/10 07:13 by nzjacic

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