User Tools

Site Tools


wiki:documentation:wiki:documentation:nicolina:12.12.19

This is an old revision of the document!


Table of Contents

12.12.19

Objectives

  • Compare growth of AML-283/284 and N2 after ~41, 45, 48 and 65 hours
  • Try 3 different exposure times using AML-283 and OP50-GFP (15,000, 17,000 and 30,000)
  • Try heat-shocking AML-283 (x20) for 1hr at 37C –> leave in 20C over the weekend
  • Update protocol
  • Meeting with Monika at 4pm
  • Set up O/N of OP50-GFP

Observations

  • AML-283 and AML-284 lay roughly 1/3 less eggs than N2 when synced
  • All 3 strains grow at about the same rate as each other for 48 hours - won't be able to observe past 48 hours due to timing and day off tomorrow
  • Binning 2 and 15,000 exposure works the best so far - Monika will try 0 binning and 30,000 exposure tomorrow
wiki/documentation/wiki/documentation/nicolina/12.12.19.1576167304.txt.gz · Last modified: 2019/12/12 17:15 by nzjacic

Bitnami