User Tools

Site Tools


wiki:documentation:wiki:documentation:nicolina:13.2.20

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

wiki:documentation:wiki:documentation:nicolina:13.2.20 [2020/02/13 17:53] (current)
nzjacic created
Line 1: Line 1:
 +====== 13.2.20 ======
  
 +===== Objectives =====
 +
 +  * Make 1XTAE from 50X stock solution
 +  * Run gel of VC3113 PCR and visualize
 +  * Order HpyCH4IV for PR671 diagnostic
 +  * Put PR671 PCR product into freezer for use when enzyme cutter arrives
 +  * Experiment with OP50 lawns
 +  * Starve GRU101
 +  * Record GRU101 with OP50
 +  * Set up OP50-GFP ON
 +  * Maintain strains
 +  * Update video log
 +
 +===== Observations =====
 +
 +  * 1XTAE - 20ml 50XTAE in 1L dH20
 +  * 100ml gel at 1.5% (1.5g agarose) with 8ul ethidium bromide (0.5ul/ml final concentration)
 +    * Gel loaded L A1 A2 ... A8 L
 +    * entire 20ul of PCR product used
 +    * 10ul for each ladder
 +    * A1 faint - was using the wrong pipette to load it so either not all of PCR product made it in there or was maybe more diffuse
 +  * GRU101
 +    * 0032 - G5, "​dab"​ lawn using soft pipette tip, 10 worms
 +      * had 4 worms going inside lawn even 0.5cm on either side and with such a tiny lawn
 +      * Monika suggests to do this with OP50-GFP but just crank up the exposure/​gain for the photo - touch the tip to the agar by hand
 +    * 0033 - G0 2ul, lawn using soft pipette tip, 10 worms
 +  * Will need to redo gel tomorrow - Tm probably too high - try 52C
 +
 +===== Gel =====
 +
 +
 +
 +===== TOMORROW =====
 +
 +  * Be sure to maintain plates 1-10 of VC3113 F3 until genotyping happens!!!!
 +  * Maintain strains
 +  * Starve GRU101
 +  * Seed "​dab"​ lawns
 +  * Record GRU101 response to "​dab"​ lawns and hopefully get longer trajectories
wiki/documentation/wiki/documentation/nicolina/13.2.20.txt ยท Last modified: 2020/02/13 17:53 by nzjacic

Bitnami