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wiki:documentation:wiki:documentation:nicolina:13.5.20 [2020/05/13 17:36] (current)
nzjacic created
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 +====== 13.5.20 + Monika Meeting Summary ======
 +
 +===== Objectives =====
 +
 +  * Take ON of OP50-RFP out of incubator
 +  * Make 10x lawn solutions
 +  * Seed 16 micro-lawns for tomorrow'​s recordings
 +  * Continue writing methods section
 +
 +===== Summary from meeting =====
 +
 +  * INF102 -
 +    * Up video duration to 10 min
 +    * See how many numbers we get and analyze
 +    * If something interesting happens and we need more numbers then can do another round of experiments --> KEEP MAINTAINING INF102!
 +  * Look up papers (see reading channel on Tuesday) - whole brain imaging - look up microscope section for description
 +  * See handbook for Zeiss and filter set i.e. RFP, GFP and YFP filters (set of drawers to the left of the Zeiss)
 +    * Filters: Emission filter bandpass
 +    * Light source - less important - lumencore LED lamp - says on side of machine
 +    * 1x on CAMERA - need to specify this since it's 32x on microscope
 +  * INF100 - TRANSFER REST OF VIDEOS ONTO MONIKA'​S COMPUTER!!! Batch run over the weekend! --> analyze videos next week
 +  * Analyzed data --> GRU101 --> all results analyzed with version 5
 +    * Don't want copies - get rid of everything v2 and below then re-run feature files using v5 - get rid of the rest
 +    * Need to do reorganizational work - want folder names to be the same as raw folder names
 +  * Continue with methods until I've done as much as I can and ask others to edit sections e.g. Luis and microscope part
 +    * Aim to complete by WEDNESDAY next week
 +  * Since results will be written as they come in then can probably help with more coding after that - still need general '​diagnostic'​ code to look at rough velocity and pumping per strain
 +
  
wiki/documentation/wiki/documentation/nicolina/13.5.20.txt ยท Last modified: 2020/05/13 17:36 by nzjacic

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