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wiki:documentation:wiki:documentation:nicolina:14.1.20 [2020/01/14 06:58] (current)
nzjacic created
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 +====== 14.1.20 ======
  
 +===== Objectives =====
 +
 +  * Sync AML-283 for Friday
 +  * Continue analyzing first 5000 frames of 0017 using Python
 +  * Post project summary onto Wiki
 +
 +===== Observations =====
 +
 +  * 0017 - only 1 trajectory found instead of 2 - one worm might be outside of the maximum but didn't have time to restart analysis using different parameters
 +    * should change max. parameters to 1000
 +    * left pharaglow to run while I'm away - should be ready for Monday
 +
 +===== While away... =====
 +
 +  * I've discussed with Jun about maintaining my strains and setting up a cross with potential successful AML-283 males today or tomorrow - **to maintain:**
 +    * AML-283
 +    * AML-284
 +    * N2 new
 +    * GRU101 (yfp)
 +    * VC3113 (tax-4)
 +    * PR671 (tax-2)
 +  * Outline for data collection for the rest of this week
 +    * **Wednesday**
 +      * Set up O/N of OP50-GFP
 +    * **Thursday**
 +      * Morning - drop 1.75ul OP50-GFP into center of an unseeded plate and allow to dry for at least 30 minutes **and** put around 60 worms onto an unseeded plate to starve for 1hr over lunch
 +      * Afternoon - run set up seen on [[wiki:protocols:foraging | this page]] to take video recordings of the worms
 +    * **Friday** - same as Thursday except if possible it would be great to collect videos using no lawn (i.e. worms are swimming in M9 until dry and then recorded)
wiki/documentation/wiki/documentation/nicolina/14.1.20.txt ยท Last modified: 2020/01/14 06:58 by nzjacic

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