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wiki:documentation:wiki:documentation:nicolina:14.2.20 [2020/02/14 16:16] (current)
nzjacic created
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 +====== 14.2.20 ======
 +
 +===== Objectives =====
 +
 +  * Starve GRU101
 +  * Experiment further with OP50-GFP lawn
 +  *<​del>​ Record GRU101</​del>​
 +  * PCR of lysed N2 to find best temperature for primers
 +    * Run gel
 +  * Run PCR of VC3113 and PR671 crosses again
 +    * <​del>​Digest PR671 PCR with restriction enzyme that arrived today</​del>​
 +  * Maintain A1-A8 and B1-B8 plates
 +  * Maintain GRU101 males
 +  * Maintain all strains
 +
 +===== Observations =====
 +
 +  * Gel showed 50C seemed to be the best temperature for the primers --> ran next PCR at 50C
 +    * Stored PCR products at -20C
 +    * No time to do digest
 +  * No time to do recordings today
 +
  
wiki/documentation/wiki/documentation/nicolina/14.2.20.txt ยท Last modified: 2020/02/14 16:16 by nzjacic

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