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wiki:documentation:wiki:documentation:nicolina:16.12.19 [2019/12/16 07:48] (current)
nzjacic created
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 +====== 16.12.19 ======
 +
 +===== Objectives =====
 +
 +  * Check heat shock of AML-283 L4s
 +  * Redo heat shock
 +  * Check on plates from weekend
 +  * Pick plates from weekend
 +  * Sync AML-283 for Thursday
 +  * Sync AML-283 and N2 in AM to compare adult-stage growth
 +  * Presentation
 +  * New Amp plate
 +  * Re-streak OP50-GFP
 +
 +===== Observations =====
 +
 +  * OP50-GFP from Friday did not grow - seems to have some contamination in it instead - need to let Amp dry for longer and re-streak
 +  * Physical nature of egg-laying defect not conclusive - should sync eggs earlier to check between 48-65 hours of development since up to 48 hours the development seems to be the same
 +  * Heat shock - some still alive! But no males... Did another heat shock
 +  * Left Amp plate to dry for a few hours - 20ul Amp + 80ul LB broth
 +  * **BEFORE CHRISTMAS**
 +    * 10cm plates --> 4 worms at L1 stage or even some eggs --> 20C
 +    * PUT 2 EMPTY AND PARAFILMED PLATES INTO 20C for JUN
 +
  
wiki/documentation/wiki/documentation/nicolina/16.12.19.txt ยท Last modified: 2019/12/16 07:48 by nzjacic

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