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Table of Contents

17.12.19

Objectives

  • Put re-streaked OP50-GFP into 4C
  • Sync AML-283
  • Presentation
  • Weekly and monthly plan

Observations

  • OP50-GFP left too long (16:30-9:00) - only 2 single colonies - streak more sparsely and leave for less time if possible
  • AML-283
    • 1/3 reduction in egg laying compared to N2
    • 7-8 hour delay till egg-laying compared to N2 - delay till adulthood?
  • AML-284
    • 1/3 reduction in egg laying compared to N2
    • Leftover unhatched eggs on plates common
    • Delay in reaching egg-laying stage - delay till adulthood?

Tomorrow

  • 9am - sync with 20 AML-283 if I can find 20 of them for 1 hr
  • 12:00 (47hrs) - CHECK AM AML-283 and N2!! –> once per hour till 17:00 (52hrs) - will be able to determine transition to adult stage
  • Check heat shock in evening
  • Set up OP50-GFP O/N
  • Pick plates

Thursday

  • Check for seeded 10cm plates

Friday

  • Check Wed sync betw. 14:00 (52hrs) and 17:00
  • Pick 4 L1 or eggs on 10cm plates
  • Put a couple parafilmed 10cm plates into 20C for Jun
wiki/documentation/wiki/documentation/nicolina/17.12.19.txt · Last modified: 2019/12/17 17:22 by nzjacic

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