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wiki:documentation:wiki:documentation:nicolina:17.12.19 [2019/12/17 17:22] (current)
nzjacic created
Line 1: Line 1:
 +====== 17.12.19 ======
  
 +===== Objectives =====
 +
 +  * Put re-streaked OP50-GFP into 4C
 +  * Sync AML-283
 +  * Presentation
 +  * Weekly and monthly plan
 +
 +===== Observations =====
 +
 +  * OP50-GFP left too long (16:​30-9:​00) - only 2 single colonies - streak more sparsely and leave for less time if possible
 +  * AML-283
 +    * 1/3 reduction in egg laying compared to N2
 +    * 7-8 hour delay till egg-laying compared to N2 - delay till adulthood?
 +  * AML-284
 +    * 1/3 reduction in egg laying compared to N2
 +    * Leftover unhatched eggs on plates common
 +    * Delay in reaching egg-laying stage - delay till adulthood?
 +
 +**Tomorrow**
 +  * 9am - sync with 20 AML-283 if I can find 20 of them for 1 hr
 +  * 12:00 (47hrs) - CHECK AM AML-283 and N2!! --> once per hour till 17:00 (52hrs) - will be able to determine transition to adult stage
 +  * Check heat shock in evening
 +  * Set up OP50-GFP O/N
 +  * Pick plates
 +
 +**Thursday**
 +  * Check for seeded 10cm plates
 +
 +**Friday**
 +  * Check Wed sync betw. 14:00 (52hrs) and 17:00
 +  * Pick 4 L1 or eggs on 10cm plates
 +  * Put a couple parafilmed 10cm plates into 20C for Jun
wiki/documentation/wiki/documentation/nicolina/17.12.19.txt ยท Last modified: 2019/12/17 17:22 by nzjacic

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