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17.2.20

Objectives

  • Maintain strains
  • Sync GRU101
  • Digest PR671 PCR
  • Run gel of PCR products
  • Put up 0028-0031 figures onto Wiki
  • Lyse N2, PR671 and VC3113 as controls for next rounds of PCR
  • Lyse JN1715, CB101 and IK105 for use as control when genotyping later
  • Seed 6cm plates

Observations

  • Set up digest of PR671 cross using this protocol:

  • 8A (VC3113) and 2B (PR671) single F2 worm lyses were successful and of the correct size
    • However Jun is suspicious about the lack of heterozygous results - decided to make lysate of most promising A and B results as well as 3 additional plates - plate lysis this time - will do PCR, digest and gel on Wednesday when I have more time
    • Lysates of controls (N2, PR671 and VC3113) were also done

Gels

L A1 A2 … A8 L

L B1 B1(digested) B2 B2D … B4 B4D L

L B5 B5D … B8 B8D L

Plan

Monday:

  • Maintain strains
  • Sync GRU101
  • Digest PRU671 PCR
  • Run gel of PCR products
  • Put up 0028-0031 figures onto Wiki
  • Lyse N2, PR671 and VC3113 as controls for next rounds of PCR
  • Lyse JN1715, CB101 and IK105 for use as control when genotyping later
  • Seed 6cm plates

Tuesday:

  • Finish 0014 manual data plotting
  • Cross CB101, JN1715 and IK105 with GRU101
  • Sync GRU101 and if possible new crosses
  • Talk with Jun about the layout of our poster
  • Chunk PR671 and VC3113 plates (1-8)

Wednesday:

  • Create BioRender figures for 0014 set up (re-read Michael Hendricks paper for illustration inspo)
  • ON OP50-GFP
  • PCR and digest of PR671 and VC3113 lysate

Thursday:

  • Seed 3x conc. lawns
  • Starve GRU101
  • Recordings of GRU101 using new lawn method (3x)
  • High-res figure of worm pumping (6-8 frames with arrow showing pump)

Friday:

  • Seed 3x conc. lawns
  • Starve GRU101
  • Recordings of GRU101 using new lawn method (3x)
  • Catch up
  • Monika meeting
wiki/documentation/wiki/documentation/nicolina/17.2.20.txt · Last modified: 2020/05/11 06:57 by nzjacic

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