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wiki:documentation:wiki:documentation:nicolina:17.2.20 [2020/02/17 08:06]
nzjacic created
wiki:documentation:wiki:documentation:nicolina:17.2.20 [2020/05/11 06:57]
nzjacic Added gel images from 17th february
Line 5: Line 5:
   * Maintain strains   * Maintain strains
   * Sync GRU101   * Sync GRU101
-  * Digest PRU671 PCR+  * Digest PR671 PCR
   * Run gel of PCR products   * Run gel of PCR products
   * Put up 0028-0031 figures onto Wiki   * Put up 0028-0031 figures onto Wiki
Line 13: Line 13:
  
 ===== Observations ===== ===== Observations =====
 +
 +  * Set up digest of PR671 cross using this protocol:
 +
 +{{ :wiki:documentation:wiki:documentation:nicolina:digest.png?400 |}}
  
   * 8A (VC3113) and 2B (PR671) single F2 worm lyses were successful and of the correct size   * 8A (VC3113) and 2B (PR671) single F2 worm lyses were successful and of the correct size
     * However Jun is suspicious about the lack of heterozygous results - decided to make lysate of most promising A and B results as well as 3 additional plates - plate lysis this time - will do PCR, digest and gel on Wednesday when I have more time     * However Jun is suspicious about the lack of heterozygous results - decided to make lysate of most promising A and B results as well as 3 additional plates - plate lysis this time - will do PCR, digest and gel on Wednesday when I have more time
     * Lysates of controls (N2, PR671 and VC3113) were also done     * Lysates of controls (N2, PR671 and VC3113) were also done
 +
 +===== Gels =====
 +
 +{{ :wiki:documentation:wiki:documentation:nicolina:as.jpg?nolink&400 |}}
 +
 +L A1 A2 ... A8 L
 +
 +{{ :wiki:documentation:wiki:documentation:nicolina:bs.jpg?nolink&400 |}}
 +
 +L B1 B1(digested) B2 B2D ... B4 B4D L
 +
 +L B5 B5D ... B8 B8D L
  
 ===== Plan ===== ===== Plan =====
wiki/documentation/wiki/documentation/nicolina/17.2.20.txt ยท Last modified: 2020/05/11 06:57 by nzjacic

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