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wiki:documentation:wiki:documentation:nicolina:17.2.20 [2020/02/17 17:09]
nzjacic added PCR digest protocol
wiki:documentation:wiki:documentation:nicolina:17.2.20 [2020/05/11 15:57] (current)
nzjacic Added gel images from 17th february
Line 5: Line 5:
   * Maintain strains   * Maintain strains
   * Sync GRU101   * Sync GRU101
-  * Digest ​PRU671 ​PCR+  * Digest ​PR671 PCR
   * Run gel of PCR products   * Run gel of PCR products
   * Put up 0028-0031 figures onto Wiki   * Put up 0028-0031 figures onto Wiki
Line 21: Line 21:
     * However Jun is suspicious about the lack of heterozygous results - decided to make lysate of most promising A and B results as well as 3 additional plates - plate lysis this time - will do PCR, digest and gel on Wednesday when I have more time     * However Jun is suspicious about the lack of heterozygous results - decided to make lysate of most promising A and B results as well as 3 additional plates - plate lysis this time - will do PCR, digest and gel on Wednesday when I have more time
     * Lysates of controls (N2, PR671 and VC3113) were also done     * Lysates of controls (N2, PR671 and VC3113) were also done
 +
 +===== Gels =====
 +
 +{{ :​wiki:​documentation:​wiki:​documentation:​nicolina:​as.jpg?​nolink&​400 |}}
 +
 +L A1 A2 ... A8 L
 +
 +{{ :​wiki:​documentation:​wiki:​documentation:​nicolina:​bs.jpg?​nolink&​400 |}}
 +
 +L B1 B1(digested) B2 B2D ... B4 B4D L
 +
 +L B5 B5D ... B8 B8D L
  
 ===== Plan ===== ===== Plan =====
wiki/documentation/wiki/documentation/nicolina/17.2.20.txt ยท Last modified: 2020/05/11 15:57 by nzjacic

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