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wiki:documentation:wiki:documentation:nicolina:18.12.19 [2019/12/18 17:38] (current)
nzjacic created
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 +====== 18.12.19 ======
 +
 +===== Objectives =====
 +
 +  *  12:00 (47hrs) - CHECK AM AML-283 and N2 once per hour
 +  * Check heat shock in evening
 +  * Set up OP50-GFP O/N
 +  * Set up OP50 O/N
 +  * Pick plates to maintain
 +
 +===== Observations =====
 +
 +  * Heat shock not yet ready - will need to wait until tomorrow or Friday to confirm
 +  * AM AML-283 compared to N2 -
 +    * AML-283 is definitely at least 5 hours behind on growth
 +    * Some eggs not hatching is definitely a concerning observation
 +    * Means I will need to try more back-crossing with N2 once I actually get male AML-283 - will probably need to sledgehammer the classical approach once back in January
 +
 +**Thursday**
 +
 +  * Seed 10cm plates using fresh OP50
 +  * Experiments in the afternoon with Monika
 +  * Seed mini-lawn with OP50-GFP
 +  * Set up OP50-GFP ​
 +
 +
  
wiki/documentation/wiki/documentation/nicolina/18.12.19.txt ยท Last modified: 2019/12/18 17:38 by nzjacic

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