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wiki:documentation:wiki:documentation:nicolina:19.11.19 [2019/11/19 16:59]
nzjacic changed plan and added to observations
wiki:documentation:wiki:documentation:nicolina:19.11.19 [2019/11/19 17:02] (current)
nzjacic note added on plate seeding
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     * However only a couple of half moon L4 hermaphrodites - from plate starvation might have gone into dauer state?     * However only a couple of half moon L4 hermaphrodites - from plate starvation might have gone into dauer state?
     * Would need to cross again earlier next time     * Would need to cross again earlier next time
 +  * Plate seeding - to make 4mm lawn I used 3.5μl of OP50 in the center of the plate - tried to keep the lawn consistently in the center by drawing the outline of the plate and putting a 4mm diameter circle in the middle - KEPT the drawing to use for dropping M9 a consistent distance away from the lawn
  
 ===== Plan ===== ===== Plan =====
wiki/documentation/wiki/documentation/nicolina/19.11.19.txt · Last modified: 2019/11/19 17:02 by nzjacic

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