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wiki:documentation:wiki:documentation:nicolina:20.11.19 [2019/11/20 08:20] (current)
nzjacic created
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 +====== 20.11.19 ======
 +
 +===== Objectives =====
 +
 +  * Test run of AML-283
 +  * Check 4mm seeded plates
 +  * Check synched AML-283/284 growth
 +  * Check other plates --> pick
 +
 +===== Observations =====
 +
 +  * Both AML-283 and AML-284 are slow growing and slow laying
 +    * Might be from fluorescent protein
 +    * New synching conditions:
 +      * 15-20x gravid adults needed for egg laying
 +      * try growing synched eggs for 72hrs
 +  * AML-284 from starved plate were mostly dauers which is why picking and egg laying were difficult
 +    * picked a new AML-284 plate
 +    * 2 egg-layers found on synching plate as well as about 20 eggs so will try my luck for Friday
 + 
 +**Microscope testing** 
 +  
 +  * Used adult worms from chunked AML-283 plate for both test runs
 +  * Distance of 2cm from lawn too far --> about 1cm from the center is enough
 +  * Will try using smaller volume when seeding next time (e.g. 2.5-3ul) - would give more leeway to start the recording and fit screen better
 +  * Test plate 2:
 +    * Gain = 0.8
 +    * worms didn't go directly onto lawn once released from M9
 +    * only 6 worms in total (1 on left and 5 on right) - couldn't find any more worms on lawn plate for some reason and was worried M9 would dry
 +    * pressed recording button too late because of having to change folder --> keep window open and ready in the corner!
 +    * 3 worms managed to reach the patch
  
wiki/documentation/wiki/documentation/nicolina/20.11.19.txt ยท Last modified: 2019/11/20 08:20 by nzjacic

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