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wiki:documentation:wiki:documentation:nicolina:21.2.20 [2020/02/21 08:47] (current)
nzjacic created
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 +====== Monika log 21.2.20 ======
 +
 +===== Accomplished =====
 +
 +  * Made figure of pumping pharynx
 +  * Made figure of video recording methodology
 +  * Made script for manually counting pumps for 0014
 +  * Tried different method of video collection where lawn is placed in the corner - unfortunately ineffective if worms placed farther than 0.5cm away from tiny lawn - seems trajectories can't be very much smaller...
 +  * Recorded videos focusing on the pharynx better at 1x magnification
 +  * Crossed CB101, JN1715 and IK105 with GRU101
 +  * Confirmed 2B as homozygous tax-2xGRU101
 +  * Put up 0028-0031 figures onto Wiki
 +
 +===== Goals =====
 +
 +  * <hi #c8bfe7>Make scale bar and time points for pumping figure - try having 15 pics 2 frames apart showing cycles of pumping - dump into visualizations folder in Dropbox when finished</hi>
 +  * <hi #c8bfe7>Complete figures by March 9th</hi>
 +  * <hi #c8bfe7>Check recordings of GRU101 (0034-0037) to see whether I can count pumping better (for 1 minute) now that pharynx should be focused</hi>
 +    * If not then recount 0014 between 700-850 - see when actual nose tip reaches lawn as well
 +  * <hi #ffc90e>Look up CB101 primers on CGC</hi>
 +  * <hi #ffc90e>Purify tax-4 PCR and send tax-4, JN1715 and IK105 for sequencing by the end of the week</hi>
 +  * <hi #ffc90e>Begin recording with tax-2 mutant</hi>
 +
 +<hi #ffc90e>Immediate</hi>
 +
 +<hi #c8bfe7>Can wait</hi>
 +
  
wiki/documentation/wiki/documentation/nicolina/21.2.20.txt ยท Last modified: 2020/02/21 08:47 by nzjacic

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