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wiki:documentation:wiki:documentation:nicolina:23.1.20 [2020/01/23 17:03] (current)
nzjacic created
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 +====== 23.1.20 ======
  
 +===== Objectives =====
 +
 +  * Take OP50-GFP out of incubator
 +  * Seed lawns for <​del>​GRU101</​del>​ AML283 recordings
 +  * Starve as amny <​del>​GRU101</​del>​ AML-283 as possible for 1hr over lunch
 +  * Record <​del>​GRU101</​del>​ AML-283 using regular settings and calibrate
 +  * Check on GRU101 HS
 +  * Finish coding analysis of Pharaglow data for 0014 - Monika will then add to overall analysis script
 +  * Begin manual counting of 0014
 +
 +===== Observations =====
 +
 +  * GRU101 - wanted to record today but will instead do AML-283 today and GRU101 first thing in the morning tomorrow
 +    * 11:15 - around 44 hours after syncing - worms seem to be at L3 stage based on size and not in this bracket by a large amount (est. 30hrs)
 +      * Will need to keep track throughout the day to adjust growth rate
 +      * To make sure they are adults by the time starving happens then would need to sync first thing in the morning on Monday and Tuesday
 +    * 15:30 - around 48hrs after syncing - reached early L4 stage now - slow by about 14hrs... takes about 62 hours for this strain to reach adulthood as well but doesn'​t seem to have the same egg-laying and hatching defects as AML-283/284
 +    * 17:00 - early L4 might have been a trick of the light - size seems to be that of worms nearing L4 now though - will need to check again at 9:00
 +  * GRU101 HS -
 +    * 2 plates show no eggs - vulval protrusion a common phenotype
 +    * 1 plate with eggs shows promise - not much vulval deformation (protrusion) and healthy looking adults
 +    * 1 plate with eggs has potential - vulval protrusion and not many eggs
 +
 +==== Tomorrow ====
 +
 +  * 9:00 seed lawns and starve GRU101 (record possible stage of growth) --> check on GRU101 HS for both me AND Jun (45min HS looking promising)
 +  * 10:30ish begin recording
 +  * 12:00 finish recording 3 GRU101 adults
 +  * 13:00 begin analyzing data if possible + finish manual counting of 0014
wiki/documentation/wiki/documentation/nicolina/23.1.20.txt ยท Last modified: 2020/01/23 17:03 by nzjacic

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