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wiki:documentation:wiki:documentation:nicolina:24.3.20 [2020/03/25 02:38] (current)
nzjacic created
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 +====== 24.3.20 ======
  
 +===== Objectives =====
 +
 +  * Debugging pharaglow
 +  * Set up ON red OP50
 +  * Aliquot antibiotics for red OP50 inoculation
 +  * Synchronize INF100 and GRU101
 +  * Take unseeded plates out of 4C
 +  * Maintain strains
 +  * Check on JN1715 cross for homozygous GFP signal
 +
 +===== Observations =====
 +
 +  * INF100 still growing very slowly - were at L2-early L4 stage yesterday and 24 hours later only a couple were egg-laying adults after incubation at 20C... Making it very difficult to time growth so that adults can be used for synchronization - maybe 10cm plates picked 3 times per week will help in increasing chances
 +  * Will want to make a figure of fluorescent pumping and pumping schematic side by side as discussed with Monika - still need a SCALE BAR that I can do in Fiji using pixel->length number Maxi collected
 +
 +===== Tomorrow =====
 +
 +  * Take red OP50 out of incubator
 +  * Seed tiny lawns in the afternoon
 +  * More pharaglow debugging
 +  * Pumping figure
wiki/documentation/wiki/documentation/nicolina/24.3.20.txt ยท Last modified: 2020/03/25 02:38 by nzjacic

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