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26.11.19

Objectives

  • Set up another sync
  • Check on sync plates from yesterday
  • Z-project images
  • Play around with ImageJ tools introduced by Monika today

Observations

  • Synced plates from yesterday seem to have worked well - no mixed stages and many of the eggs hatched
  • Plates from today synced at 14:30
  • ImageJ
    • Cropped image –> image –> duplicate
    • Image –> adjust –> threshold - get a good outline of only the worm
    • Analyze –> analyze particles –> set size e.g. 100-infinity (can filter which worm to track using size rather than physically having to crop them out)
    • Analyze –> set measurements –> check al boxes for now
    • See whether any of the parameters best describe pumping action
      • XM?
      • YM?
      • Perim.?
  • Z-project settings: 1800, 500, 200
wiki/documentation/wiki/documentation/nicolina/26.11.19.txt · Last modified: 2019/11/26 16:13 by nzjacic

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