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wiki:documentation:wiki:documentation:nicolina:27.11.19 [2019/11/27 17:20] (current)
nzjacic created
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 +====== 27.11.19 ======
  
 +===== Objectives =====
 +
 +  * Learn about python with Monika --> set up code for graphically analyzing pumps
 +  * Set up last sync of the week
 +  * Pick bleached plates of AML-283/284
 +  * Collect data using syncs from Monday - PUT ON HOLD TILL TOMORROW DUE TO SLOW GROWTH
 +  * Determine number of hours needed for AML-283/284 to reach ideal adult stage...
 +
 +
 +===== Syncing =====
 +
 +  * AML-283
 +    * 11:00 - BA
 +    * 12:00 - BA
 +    * 13:00 - BA
 +    * 14:00 - BA
 +    * 15:00 - good stage? (49 hours)
 +  * AML-284
 +    * 11:00 - BA
 +    * 12:00 - BA
 +    * 13:00 - BA
 +    * 14:00 - BA
 +  * AML283 sync time: 14:00
 +  * AML284 sync time: 16:30
 +
 +===== Observations =====
 +
 +  * AML-283 sync failed because not enough eggs laid - tried to set it up again - didn't work again - many seemed to have a podruding vulva (hot picking, spontaneous mutation, etc.) --> got rid of plate from Tuesday and chunked plate from last weekend to see if problem persists ​
 +  * Egg laying and growth of both AML-283/284 has been confirmed to be very slow and gonad growth slightly unusual - will leave sync from Monday an extra day and begin data collection again tomorrow
 +  * Set up an N2 control sync to see whether egg laying and growth time affected
 +    * Yes --> something wrong with plates and buffers
 +    * No --> something wrong with the strain
 +  * Will need to make stock solutions and plates again from scratch tomorrow morning just in case - can receive autoclave training as well
 +
 +===== Plan =====
 +
 +**Thursday**
 +
 +  * Morning
 +    * 8:30-10:00 German
 +    * Check growth of sync plate from Monday
 +    * Collect data from plate
 +  * Afternoon
 +    * Redo stock solutions
 +    * Analyze video 0002 using ImageJ and Python
 +
 +**Friday**
 +
 +  * Morning
 +    * Check sync plate from Tuesday
 +    * Collect data from plate
 +  * Afternoon
 +    * Continue analyzing videos
 +    * Set up syncs of AML283/284 and N2 at the same time and let grow over the weekend
wiki/documentation/wiki/documentation/nicolina/27.11.19.txt ยท Last modified: 2019/11/27 17:20 by nzjacic

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