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wiki:documentation:wiki:documentation:nicolina:27.2.20 [2020/02/27 07:55] (current)
nzjacic created
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 +====== 27.2.20 ======
  
 +===== Objectives =====
 +
 +  * Maintain strains
 +    * CB101 and IK105 crosses also put into 15C because worms were too small to transfer onto 10cm plates
 +    * All maintenance strains as well as new INF100 put on 10cm plates at 20C
 +    * Gravid hermaphrodites from GRU101 male cross were transferred onto 10cm plate while original 6cm plate with already-laid eggs was put at 15C
 +    * 1A-8A were picked onto 10cm plates and stored at 20C
 +  * Count manual pumping of 4 worms:
 +    * worm1 - 0034
 +    * worm2 - 0037
 +    * worm3 - 0035 (top)
 +    * worm4 - 0035 (side)
 +
 +===== Goals =====
 +
 +  * Purify tax-4 PCR
 +  * PCR of CB101, tax-4 and IK105 (Wednesday will be genotyping day) --> send strains for sequencing
 +  * Gel of tax-4 plate lysis using new primer within deleted region
 +  * Continue watching JN1715 - see if F3s tell me anything
 +  * Finish figures that I didn't finish this week
 +  * Learn about microscopes on Tuesday from Luis
 +  * Sync INF100 and GRU101 and see if I have time to record videos this week (maybe just a couple of tests) - if not then can leave all recording for next week
wiki/documentation/wiki/documentation/nicolina/27.2.20.txt ยท Last modified: 2020/02/27 07:55 by nzjacic

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