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wiki:documentation:wiki:documentation:nicolina:3.3.20 [2020/03/04 06:30] (current)
nzjacic created
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 +====== 3.3.20 ======
 +
 +===== Objectives =====
 +
 +  * Plate lysis of VC3113 cross A1-A4
 +  * Plate lysis of CB101 and IK105 cross
 +  * Check JN1715 F3
 +  * Sync INF100 (2 plates) and GRU101 (1 plate) for potential recording on Friday
 +  * Cross GRU101 males
 +  * Maintain A1-8
 +  * Maintain strains
 +  * Give Monika positional information of manually counted worms and frame where nose first touches the lawn
 +  * IT ticket asking for a VPN to access Monika's computer remotely
 +  * Purify A5-8 PCR and prepare for sequencing
 +
 +===== Observations =====
 +
 +  * All 20 JN1715 F2 plates are heterozygotes - will need to pick 50 individual F3 once plates become available
 +  * Primers not arrived yet so will need to see whether I can do genotyping tomorrow or not
 +  * Preparing for sequencing notes:
 +    * Must be in 1.5ml eppendorf
 +    * Put bar code onto eppendorf before sending off
 +    * 15ul of DNA required but single-tube PCR purification only produces 7ul - top up with 8ul of water
 +    * Add 2ul of 10um F or R primer depending on where point mutation is positioned - F is better because you don't have to go through reverse complementation 
 +
  
wiki/documentation/wiki/documentation/nicolina/3.3.20.txt ยท Last modified: 2020/03/04 06:30 by nzjacic

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