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wiki:documentation:wiki:documentation:nicolina:4.3.20 [2020/03/04 06:02]
nzjacic created
wiki:documentation:wiki:documentation:nicolina:4.3.20 [2020/03/04 09:22] (current)
nzjacic Added presentation making and plan
Line 9: Line 9:
   * Seed more plates to pick 50 F3 from JN1715 cross tomorrow   * Seed more plates to pick 50 F3 from JN1715 cross tomorrow
   * Won't be recording tomorrow due to time constraints   * Won't be recording tomorrow due to time constraints
 +  * Create presentation for Friday
  
 ===== Observations ===== ===== Observations =====
Line 20: Line 21:
   * 1C lysate missing after putting back into ice bucket   * 1C lysate missing after putting back into ice bucket
  
 +===== Tomorrow =====
  
 +  * 10:00 make 3 gels for PCR analysis - take PCR products out of the freezer
 +  * 10:45 load gels
 +  * 11:00 run gels - ask Monika about presentation
 +  * 12:00 stop gels before lunch
 +  * 13:00 visualize gels
 +  * 13:30 purify successful products - in the mean time prepare sequencing tubes
 +  * 14:15 create sequencing solutions
 +  * 14:30 send tubes off for sequencing
 +  * 15:00 microscope building with Luis
 +  * 16:00 separate 50 F3 JN1715 and maintain C1-8 and D1-8
wiki/documentation/wiki/documentation/nicolina/4.3.20.txt ยท Last modified: 2020/03/04 09:22 by nzjacic

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