User Tools

Site Tools


wiki:documentation:wiki:documentation:nicolina:6.2.20

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

wiki:documentation:wiki:documentation:nicolina:6.2.20 [2020/02/06 08:11] (current)
nzjacic created
Line 1: Line 1:
 +====== 6.2.20 ======
 +
 +===== Objectives =====
 +
 +  * Maintain worms
 +  * Maintain GRU101 males
 +  * Check F1 from VC3113 and PR671
 +    * Set up single L4 of both crosses to grow for Monday
 +  * Continue analysis on 0028-0031 using updated pharaglow code
 +  * ON OP50-GFP
 +  * Set up 0025 to analyze using new pharaglow before leaving
 +
 +===== Observations =====
 +
 +  * 0028-0031 (gain 0)
 +    * 0028 - lawn too prominent in the background even if subtraction up to 50
 +    * 0029 - same as above
 +    * 0030 - same as above - changing min size or length doesn't help
 +    * 0031 - highlights importance of having a neat lawn - will need to ask Jun about flexible pipette tips tomorrow and repeat 1x conc. data - gain seems to be necessary to distinguish worms from background better
 +  * 0025 (gain 5) 
 +    * Works very well - much better than under old pharaglow
 +
 +===== Tomorrow =====
 +
 +  * Starve GRU101
 +  * Seed 1x lawns
 +  * Record GRU101
 +  * Try combining 0014 and 0025 data to test multiple video code
 +  * Monika Log for meeting
  
wiki/documentation/wiki/documentation/nicolina/6.2.20.txt ยท Last modified: 2020/02/06 08:11 by nzjacic

Bitnami