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wiki:documentation:wiki:documentation:nicolina:9.12.19 [2019/12/09 17:59] (current)
nzjacic created
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 +====== 9.12.19 ======
 +
 +===== Objectives =====
 +
 +  * Set up syncs of AML-283, AML-284 and N2 to compare growth on new plates
 +  * Pick AML-283, AML-284 and N2 from weekend plates to maintain
 +  * Collect 006 data
 +
 +===== Observations =====
 +
 +  * Weekend plates
 +    * AML-283 - quite synchronized plate - only 3 egg-laying adults available, rest were L4 - makes sense if only L4 picked on Friday - **in the future** would need to pick L4 on Thursday and leave to grow over the weekend with at least 100ul seeded onto plate - only 3 adults used for sync
 +    * AML-284 - further along than AML-284 but fewer in numbers - only 7 used for sync
 +    * N2 - many egg-laying adults present - was able to pick a full 15
 +  * Analyze --> Tools --> Synchronize window - can synchronize oval selection (NOT brush selection) across as many windows as I have open - can make a crude outline of the lawn - **ask Monika whether an overlay in each video is better and more accurate** - good enough for now while taking manual data
 +  * Criteria -
 +    * Worm hits the lawn when the tip of the mouth first touches the outer line of the drawn lawn ring (grey)
 +    * Full pump = one opening and closing of the grinder
 +    * Excel spreadsheet recorded when grinder was opened fully (ie when TB looked the widest)
  
wiki/documentation/wiki/documentation/nicolina/9.12.19.txt ยท Last modified: 2019/12/09 17:59 by nzjacic

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