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wiki:documentation:wiki:documentation:nicolina:bleaching_and_other_notes [2019/11/11 15:42]
nzjacic created
wiki:documentation:wiki:documentation:nicolina:bleaching_and_other_notes [2019/11/14 17:39]
nzjacic inserted picture
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 Need lots of worms - otherwise you risk losing them in the process... Need lots of worms - otherwise you risk losing them in the process...
 +
 +Finish by removing excess liquid after overnight till just under 0.1ml line --> pipette the remaining liquid up and down 3 times before transferring the liquid onto the the non-contaminated 6cm plate
 +
 +**For spot bleaching:​**
 +
 +  - Add 50-100ul bleach to the corner of the uncontaminated plate away from the bacterial lawn
 +  - Pick up 5-6 gravid worms and transfer onto bleach
 +  - Shake plate a bit to separate worms
 +  - Incubate O/N at 20C
  
  
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   * Amount of bleaching done at one time recommended = 6 different strains or less - otherwise difficult to keep track of all of them   * Amount of bleaching done at one time recommended = 6 different strains or less - otherwise difficult to keep track of all of them
   * Filter tips are more useful to minimize cross-contamination   * Filter tips are more useful to minimize cross-contamination
 +
 +{{ :​wiki:​documentation:​wiki:​documentation:​nicolina:​bleaching.png?​600 |}}
  
 ===== Other notes ===== ===== Other notes =====
wiki/documentation/wiki/documentation/nicolina/bleaching_and_other_notes.txt ยท Last modified: 2019/11/14 17:39 by nzjacic

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