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Freezing worms

  • L1/L2 the best for freezing - have the highest survival rate
  • Want a borderline starved plate
  • Keep plates after washing - don't know if freezing worked the first time
  • Set up one tube as a thaw control - thaw it after leaving in -80C overnight
  • Use around 5ml of freezing solution to wash plates –> aliquot 1ml into screw cap tubes –> place in ice for around 30 min –> transfer to -80C freezer
wiki/documentation/wiki/documentation/nicolina/freezing_worms.txt · Last modified: 2019/11/25 17:10 by nzjacic