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wiki:documentation:wiki:documentation:nicolina:freezing_worms [2019/11/25 17:10] (current)
nzjacic created
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 +====== Freezing worms ======
 +
 +=====  =====
 +
 +  * L1/L2 the best for freezing - have the highest survival rate
 +  * Want a borderline starved plate
 +  * Keep plates after washing - don't know if freezing worked the first time
 +  * Set up one tube as a thaw control - thaw it after leaving in -80C overnight
 +  * Use around 5ml of freezing solution to wash plates --> aliquot 1ml into screw cap tubes --> place in ice for around 30 min --> transfer to -80C freezer
 +
 +
  
wiki/documentation/wiki/documentation/nicolina/freezing_worms.txt ยท Last modified: 2019/11/25 17:10 by nzjacic

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