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wiki:documentation:wiki:documentation:nicolina:monik_log_24.1.20 [2020/01/24 06:15] (current)
nzjacic created
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 +====== Monika log 24.1.20 ======
  
 +===== Accomplished this week =====
 +
 +  * Jun has established that AML-283 decreases in fluorescence when back-crossed to N2 (can be seen starting with F1)
 +      * Jun is attempting to figure out whether AML-284 has the same decrease in fluorescence with each N2 cross generation
 +      * GRU101 (YFP strain) has been established as the next candidate for recording
 +  * Attempted heat shocking GRU101 for the first time to isolate males
 +  * Recorded GRU101 for the first time
 +    * 1x lawn
 +    * 10x lawn
 +    * No lawn
 +  * Established GRU101 growth time to adulthood (67-68hrs) and that there are no immediate egg-laying/hatching defects
 +  * VNC now connects my computer to Monika's - I can run Python Notebooks on recordings from my computer
 +  * I've contributed to code!
 +    * Velocity calculated for all worm trajectories
 +    * Lawn filled and binarized
 +    * List of whether worm is inside or outside the lawn in any given frame
 +  * Manual pumping data collected for 0014 to calibrate automatic data collection
 +
 +===== Goals =====
 +
 +  * Code velocity half of the plot (before and after worms enter the lawn) - want to take an average of the velocities
 +  * Continue calibrating data recordings (gain still seems to be too high for AML-283 - might need to put gain down to 0 for GRU101)
 +  * Continue trying to find GRU101 male to begin crossing
 +
 +===== Plan =====
 +
 +**Monday:**
 +
 +  * Sync GRU101
 +  * Check on HS
 +    * If HS unsuccessful then set up another
 +    * If successful then begin crossing with N2
 +  * Coding
 +  * Maintain plates
 +  * Make more NGM media
 +
 +**Tuesday:**
 +
 +  * Sync GRU101
 +  * Coding
 +
 +**Wednesday:**
 +
 +  * Coding
 +  * ON OP50-GFP
 +
 +**Thursday:**
 +
 +  * Seed lawns (1x)
 +  * Calibrate GRU101 recording
 +  * ON OP50-GFP
 +
 +**Friday:**
 +
 +  * Seed lawns (1x)?
 +  * More recording?
 +  * ON OP50-GFP
 +  * Check on cross or check on HS
wiki/documentation/wiki/documentation/nicolina/monik_log_24.1.20.txt ยท Last modified: 2020/01/24 06:15 by nzjacic

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