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wiki:documentation:wiki:documentation:nicolina:monika_log_14.2.20 [2020/02/14 16:26] (current)
nzjacic created
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 +====== Monika log 14.2.20 ======
 +
 +===== Accomplished =====
 +
 +  * Combined 0028-0031 data
 +  * Found best method for creating small lawns - dip hard pipette tip into 3x conc OP50-GFP and dab gently onto agar --> increase exposure to 300,000 to capture lawn
 +  * PCR ready for genotyping VC3113/​PR671xGRU101 crosses
 +  * Ordered restriction enzyme for PR671 genotyping and it arrived today
 +  * Velocity of 0014 plotted and started on code for manual pumping
 +  * Made primers for IK105 and JN1715 which arrived this week
 +
 +===== Goals =====
 +
 +  * Finish 0014 manual data plotting
 +  * Create BioRender figures for 0014 set up (re-read Michael Hendricks paper for illustration inspo)
 +  * Cross CB101 with GRU101 - see cgc description of CB101 for physical recognition of strain
 +  * High-res figure of worm pumping (6-8 frames with arrow showing pump)
 +  * Talk with Jun about the layout of our poster
 +  * Put up 0028-0031 figures onto Wiki
 +  * Start thinking about paper intro
 +  * Finish genotyping
 +  * Get more recordings using new lawn method and strains
 +
 +
  
wiki/documentation/wiki/documentation/nicolina/monika_log_14.2.20.txt ยท Last modified: 2020/02/14 16:26 by nzjacic

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