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wiki:documentation:wiki:documentation:nicolina:monika_log_22.11.19

Monika log 22.11.19

Accomplished this week

  • Began practicing how to sync
  • Determined ideal volume to seed 4mm lawns using new OP50 that arrived this week
  • Took first videos using experimental protocol
  • Began analyzing videos using Fiji

Goals

  • Get AutoROI (Time Series Analyzer plugin) to work - for some reason there is no time integration, points are put onto the same slice so no interpolation can happen (?)
  • Set up synches on Monday, Tuesday and Wednesday for data collection Wednesday, Thursday and Friday - adjust recording settings and practice setting up plates for analysis
    • Also determine the ideal number of hours till adulthood - want somewhere between borderline adult (dark grey and small - takes about 46 hours) and laying of 1st eggs (eggs seen on plate)
    • MAKE SURE ALL ADULTS ARE TAKEN AWAY AND NO EGGS ARE TRANSFERRED!!
  • Make a GitHub account and send details to Monika so she can share Anaconda/Python analysis with me
  • By mid-next week should be able to tell whether Fiji can help me determine when a pump happens (judge differences in intensities or other parameters)
  • Z-stack projection - increase spacing a little (e.g. 11 or 12 instead of increment of 10) and crop individual worms (want just before the lawn is reached and a little bit afterwards) - would make a nice preliminary figure as proof of principle that slowing down does happen once they reach the lawn
    • Mark the outline of the lawn as well - either with a red line or by overlaying the picture of the lawn taken before recording took place
wiki/documentation/wiki/documentation/nicolina/monika_log_22.11.19.txt · Last modified: 2019/11/22 16:41 by nzjacic

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