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wiki:documentation:wiki:documentation:nicolina:monika_log_22.11.19 [2019/11/22 16:41] (current)
nzjacic created
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 +====== Monika log 22.11.19 ======
 +
 +===== Accomplished this week =====
 +
 +  * Began practicing how to sync
 +  * Determined ideal volume to seed 4mm lawns using new OP50 that arrived this week
 +  * Took first videos using experimental protocol
 +  * Began analyzing videos using Fiji
 +
 +===== Goals =====
 +
 +  * <hi #​ffc90e>​Get AutoROI (Time Series Analyzer plugin) to work - for some reason there is no time integration,​ points are put onto the same slice so no interpolation can happen (?​)</​hi>​
 +  * Set up synches on Monday, Tuesday and Wednesday for data collection Wednesday, Thursday and Friday - adjust recording settings and practice setting up plates for analysis
 +    * Also determine the ideal number of hours till adulthood - want somewhere between borderline adult (dark grey and small - takes about 46 hours) and laying of 1st eggs (eggs seen on plate)
 +    * MAKE SURE ALL ADULTS ARE TAKEN AWAY AND NO EGGS ARE TRANSFERRED!!
 +  * Make a GitHub account and send details to Monika so she can share Anaconda/​Python analysis with me
 +  * By mid-next week should be able to tell whether Fiji can help me determine when a pump happens (judge differences in intensities or other parameters) ​
 +  * Z-stack projection - increase spacing a little (e.g. 11 or 12 instead of increment of 10) and crop individual worms (want just before the lawn is reached and a little bit afterwards) - would make a nice preliminary figure as proof of principle that slowing down does happen once they reach the lawn
 +    * Mark the outline of the lawn as well - either with a red line or by overlaying the picture of the lawn taken before recording took place
 +
 +
  
wiki/documentation/wiki/documentation/nicolina/monika_log_22.11.19.txt ยท Last modified: 2019/11/22 16:41 by nzjacic

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