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Monika log 29.11.19

Accomplished this week

  • Pipeline developed in ImageJ to automatically put data into ROI manager (REMEMBER: need to click on original video, not thresholded video)
  • Made z-projections for two worms with inversion and lawn outline
  • Collected 3 more sets of videos for both AML-283 and AML-284
  • Created a python pipeline analyzing video data for pumps
  • Maintaining bleached and uncontaminated AML-283 and AML-284
  • Identified slow growth in AML-283/284 and need to troubleshoot why

Goals

  • Seed plates with smaller lawns (try 2.5ul) - test how this would look first at 32X magnification
  • Troubleshoot what's causing the slow growth
    • Set up sync on Monday with N2 as a control
    • Redo plates and solutions on Monday
  • Collect data on 3 worms 5 seconds before and after entering the lawn (0004, 0005):
    1. Frames with pumps manually –> compare to frames where pumping may occur using python pipeline
    2. Frames entering the lawn manually
  • Create more videos for AML-283/284 - begin homogenizing video settings and try at 32X MAGNIFICATION instead of 22.5X - want to be able to resolve worms further
    • Monday evening –> Thursday morning
    • Tuesday evening –> Friday morning
  • Reading on smell, chemosensation and food sensing neurons we might want to order mutants and rescues of for pre-emptive pumping experiments by Wednesday meeting 1-2pm
  • Presentation

Plan

Monday:

  • Morning
    • Make new plates and solutions
    • Autoclave training
    • Check new seeding size under microscope
    • People coming to install the gas
  • Afternoon
    • Set up sync of AML-283/284 and N2 from bleached weekend plate
    • Pick plates from weekend
    • Pour more 6cm plates for tiny lawn seeding

Tuesday:

  • Morning
    • Reading for Wednesday
  • Afternoon
    • Set up sync of AML-283/284 and N2 from bleached weekend plate
    • Seed tiny lawns

Wednesday:

  • Morning
    • Presentation preparation
    • 11:00 occupational health and safety
  • Afternoon
    • Monica meeting
    • Pick AML-283/284

Thursday:

  • Morning
    • Microscope video collection
    • 0004 and 0005 manual data analysis (bring headphones)
    • Check growth comparison with N2
  • Afternoon
    • Presentation preparation

Friday:

  • Morning
    • Microscope video collection
    • Presentation
    • Check growth comparison with N2
  • Afternoon
    • 0004 and 0005 manual data analysis or new video data analysis
wiki/documentation/wiki/documentation/nicolina/monika_log_29.11.19.txt · Last modified: 2019/11/29 16:21 by nzjacic

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