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wiki:documentation:wiki:documentation:nicolina:monika_log_7.2.20 [2020/02/07 17:17] (current)
nzjacic created
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 +====== Monika log 7.2.20 ======
  
 +===== Accomplished =====
 +
 +  * Cleaned up code used for analyzing pharaglow results e.g. Markdowns and descriptive comments
 +  * Made code for and graph the 4 data sets collecting 1x conc data with starved GRU101 but not yet tested
 +  * Changed figure axes units into time in seconds and velocity in um/s
 +  * Uploaded notebooks and old pharaglow results from analysis to Dropbox
 +  * Switched to using updated pharaglow - analyzed 0014 and 0025
 +  * Labeled in log which movies don't need to be consulted any more and can be compressed
 +  * Began crossing schedule with //tax-2// and //tax-4//
 +    * 6 x single F1 left to grow over the weekend for both strains
 +  * Tried 5ul of 5x from yesterday to make smaller and more concentrated lawns
 +    * Too viscous to pull into tip - got it in by chance and lawn still a bit too big
 +      * Try 2.5 ul at 3x concentration
 +    * Less prominent poking though
 +  * Started maintaining IK105 (AWA/AWC) and JN1715 (AWB)
 +    * IK105 chunked - if 2 adults or less grow on Monday then will need to defrost them
 +    * JN1715 - needs bleaching
 +  * Started maintenance of CB101 (unc9) - no gap junctions
 +
 +===== Goals =====
 +
 +  * Continue troubleshooting to combine 0028-0031 data
 +  * Collect more videos of no lawn and more concentrated lawns to get higher sample sizes using GRU101 while the other strains are crossing
 +    * **CHANGES TO VIDEOS**:
 +      * G5 for videos
 +      * transfer starved GRU101 into M9 for quicker transfer
 +      * Difference between putting 10 worms or 20 worms doesn'​t make a huge degree of difference - may as well use 10 worms to record more videos
 +      * smaller lawn sizes and higher concentration
 +      * use faster SSD drive
 +  * Genotype GRU101xVC3113 and GRU101xPR671 by the end of the week
 +  * Graph velocity and pumping of manual 0014 data
 +  * Check when most entries into lawn happen - maybe can make videos even shorter
 +  * Make primers for IK105 and JN1715
 +
 +===== Plan =====
 +
 +**Monday:**
 +
 +  * Morning
 +    * Check IK105 chunk
 +    * Check F2 of tax-2/4 crosses --> genotyping plates
 +    * Maintain strains and crosses
 +  * Afternoon
 +    * Sync GRU101
 +    * Make Amp plate and let dry
 +  * Goal: create primers for IK105 and JN1715
 +
 +**Tuesday:​**
 +
 +  * Morning
 +    * Continue plotting manual 0014 data
 +  * Afternoon
 +    * Sync GRU101
 +    * Re-streak OP50-GFP
 +    * Pharaglow 0028
 +
 +**Wednesday:​**
 +
 +  * Morning
 +    * Genotype single F2s if ready - ask Jun how
 +    * Pharaglow 0029
 +  * Afternoon
 +    * Continue plotting manual 0014 data
 +    * Troubleshoot multiple video code using 0014 and 0025
 +    * ON OP50-GFP
 +    * Pharaglow 0030
 +
 +**Thursday:​**
 +
 +  * Morning
 +    * Starve as many worms as possible
 +    * Seed new lawns
 +    * Begin recordings
 +    * Pharaglow 0031
 +  * Afternoon
 +    * Try putting together 0028-0031
 +    * ON OP50-GFP
 +    * Maintain
 +
 +**Friday:**
 +
 +  * Morning
 +    * Starve as many worms as possible
 +    * Seed new lawns
 +    * Begin recordings
 +  * Afternoon
 +    * Debug and catch up
 +    * Maintain
wiki/documentation/wiki/documentation/nicolina/monika_log_7.2.20.txt ยท Last modified: 2020/02/07 17:17 by nzjacic

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