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wiki:documentation:wiki:documentation:nicolina:notes_from_today [2019/11/05 10:28]
nzjacic created
wiki:documentation:wiki:documentation:nicolina:notes_from_today [2019/11/05 13:02]
nzjacic
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 ====== Notes from today ====== ====== Notes from today ======
 +
 +===== OP50 and sterile technique =====
  
   * No selection for OP50 - need to have VERY good sterile technique to avoid contamination   * No selection for OP50 - need to have VERY good sterile technique to avoid contamination
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   * Use less OP50 culture when worm crosses (male and female mating) is desired   * Use less OP50 culture when worm crosses (male and female mating) is desired
   * Prevent spreading OP50 culture to very edge of 3.5 and 6cm plates   * Prevent spreading OP50 culture to very edge of 3.5 and 6cm plates
 +
 +===== Seeding 6cm plates =====
 +
 +  * Contamination can be seen by eye
 +  * Pipette culture directly into the center
 +  * Leave overnight to dry - alternatively under time pressure can leave in fume hood with lid slightly cracked for an hour
  
  
  
  
wiki/documentation/wiki/documentation/nicolina/notes_from_today.txt ยท Last modified: 2019/11/06 15:16 by nzjacic

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