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wiki:protocols [2019/12/04 11:08]
jliu [How to stage C. elegans]
wiki:protocols [2020/03/26 11:32] (current)
jliu [Worm M9 buffer]
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 Alternatively:​ Alternatively:​
- For 1 liter, dissolve 136.1 g KH<​sub>​2</​sub>​PO<​sub>​4</​sub>​ in about 800 ml dH<​sub>​2</​sub>​O,​ then adjust to pH 6.0 with solid KOH (approx 15 g) before bringing up to volume. Make 100 ml aliquots and autoclave.+ For 1 liter, dissolve 136.1 g KH<​sub>​2</​sub>​PO<​sub>​4</​sub>​ in about 800 ml dH<​sub>​2</​sub>​O,​ then adjust to pH 6.0 with solid KOH (approx 15 g) before bringing up to volume. Make 100 ml aliquots and autoclave. Jun's note: you may also use ~53ml of 5M KOH solution.
  
 ==== Nematode growth medium (NGM) agar ==== ==== Nematode growth medium (NGM) agar ====
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   *  1 ml autoclaved/​sterile 1 M MgSO<​sub>​4</​sub>​.   *  1 ml autoclaved/​sterile 1 M MgSO<​sub>​4</​sub>​.
   
- +Jun's note: you can also make 10X M9 solution by X10 of each components.
 ==== Worm lysis solution ==== ==== Worm lysis solution ====
 This makes 100 ml 2x worm lysis solution. It is used for worm egg synchronization. This makes 100 ml 2x worm lysis solution. It is used for worm egg synchronization.
wiki/protocols.1575454123.txt.gz ยท Last modified: 2019/12/04 11:08 by jliu

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