Scholz Lab Wiki

Parameters for needle pulling

Borosilicate glass
ITEM#: BF100-50-10

Heat 526
Pull 80
Vel 120
del 150

Stored in program 1

With these parameters, the needle seems to give the best performance in my hand.

Recovery buffer: “After injection, animals were teased off the pad, and placed in 30 ml of recovery buffer (5 mM HEPES pH 7.2, 3 mM CaCl2, 3 mM MgCl2, 66 mM NaCl, 2.4 mM KCl, 4% glucose) on NGM plates.”
This is adapted from: Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange
Michael Nonet; Genetics, Volume 215, Issue 4, 1 August 2020, Pages 903–921
https://academic.oup.com/genetics/article-abstract/215/4/903/6065543?redirectedFrom=fulltext